functional analysis of the rice ap3 homologue osmads16 by rna interference
文献类型：期刊论文
| 作者 | Xiao H ; Wang Y ; Liu DF ; Wang WM ; Li XB ; Zhao Xianfeng ; Xu JC ; Zhai WX ; Zhu LH |
| 刊名 | Plant Molecular Biology
 |
| 出版日期 | 2003 |
| 卷号 | 52期号:5页码:957-966 |
| 关键词 | AP3 double-stranded RNA floral organ identity OsMADS16 rice (Oryza sativa L.) RNA interference FLORAL-ORGAN IDENTITY DOUBLE-STRANDED-RNA MADS-BOX GENES ECTOPIC EXPRESSION ARABIDOPSIS-THALIANA GENOMIC ANALYSIS HOMEOTIC GENES ANTISENSE RNA CLASS-B PLANTS |
| 通讯作者 | Zhu, LH (通讯作者), Chinese Acad Sci, Inst Genet & Dev Biol, 917 Bld,Datun Rd, Beijing 100101, Peoples R China |
| 收录类别 | SCI |
| 公开日期 | 2010-08-25 |
| 附注 | The rice OsMADS16 gene is phylogenetically related to the angiosperm B-function MADS-box genes. To investigate if OsMADS16 functions as an AP3/DEF orthologue to regulate the development of lodicules and stamens in rice, we isolated its genomic sequences and characterized its functions in planta by RNA interference. The genomic sequence of the OsMADS16 gene shows that it shares high similarity in genomic structure and the deduced amino acid sequence with the maize B-class gene, Si1. Transgenic lines from the introduced gene expressing double-stranded RNA with the OsMADS16 cDNA fragment were male-sterile and displayed alternations of lodicules and stamens, occasionally depressed palea and overgrown glume. The two lodicules were converted into four palea/lemma-like organs and some stamens into carpels. Further investigations of the transcription of OsMADS16 gene in these transgenic lines by RT-PCR revealed that its transcript was significantly reduced. Transcription of a rice PI homologous gene, OsMADS4, was also reduced remarkably in the transgenic plants. Our results demonstrate that OsMADS16 is an AP3/DEF orthologue to specify the identities of lodicules and stamens in rice flower and also support that OsMADS4 is a PI orthologue. In addition, these results suggest that RNA interference is a useful tool for functional genomics in rice. |
| 源URL | [http://124.16.136.157/handle/311060/4978]  |
| 专题 | 软件研究所_信息安全国家重点实验室_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xiao H,Wang Y,Liu DF,et al. functional analysis of the rice ap3 homologue osmads16 by rna interference[J]. Plant Molecular Biology,2003,52(5):957-966. |
| APA | Xiao H.,Wang Y.,Liu DF.,Wang WM.,Li XB.,...&Zhu LH.(2003).functional analysis of the rice ap3 homologue osmads16 by rna interference.Plant Molecular Biology,52(5),957-966. |
| MLA | Xiao H,et al."functional analysis of the rice ap3 homologue osmads16 by rna interference".Plant Molecular Biology 52.5(2003):957-966. |
入库方式： OAI收割
来源：软件研究所
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