Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models
文献类型：期刊论文
| 作者 | Liu, Wenjing1,2; Sun, Zhaotian3; Chen, Jinyu4; Jing, Chuanbo3 |
| 刊名 | JOURNAL OF SPECTROSCOPY
 |
| 出版日期 | 2016 |
| 页码 | 6 |
| ISSN号 | 2314-4920 |
| DOI | 10.1155/2016/1603609 |
| 英文摘要 | Raman spectra of human colorectal tissue samples were employed to diagnose colorectal cancer. High-quality Raman spectra were acquired from normal and cancerous colorectal tissues from 81 patients. Subtle Raman variations, such as for peaks at 1134 cm(-1) (protein, C-C/C-N stretching) and 1297 cm(-1) (lipid, C-H-2 twisting), were observed between normal and cancerous colorectal tissues. The average peak intensity at 1134 and 1297 cm(-1) was increased from approximately 235 and 72 in the normal group, respectively, to 315 and 273 in the cancer group. The variations of Raman spectra reflected the changes of cell molecules during canceration. The multivariate statistical methods of principal component analysis-linear discriminant analysis (PCA-LDA) and partial least-squares-discriminant analysis (PLS-DA), together with leave-one-patient-out cross-validation, were employed to build the discriminationmodel. PCA-LDA was used to evaluate the capability of this approach for classifying colorectal cancer, resulting in a diagnostic accuracy of 79.2%. Further PLS-DA modeling yielded a diagnostic accuracy of 84.3% for colorectal cancer detection. Thus, the PLS-DA model is preferable between the two to discriminate cancerous from normal tissues. Our results demonstrate that Raman spectroscopy can be used with an optimized multivariate data analysis model as a sensitive diagnostic alternative to identify pathological changes in the colon at the molecular level. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB14020201] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000390153900001 |
| 出版者 | HINDAWI PUBLISHING CORP |
| 源URL | [http://ir.amss.ac.cn/handle/2S8OKBNM/24394]  |
| 专题 | 中国科学院数学与系统科学研究院 |
| 通讯作者 | Jing, Chuanbo |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.4 Hosp, Jinan 250031, Peoples R China 4.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wenjing,Sun, Zhaotian,Chen, Jinyu,et al. Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models[J]. JOURNAL OF SPECTROSCOPY,2016:6. |
| APA | Liu, Wenjing,Sun, Zhaotian,Chen, Jinyu,&Jing, Chuanbo.(2016).Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models.JOURNAL OF SPECTROSCOPY,6. |
| MLA | Liu, Wenjing,et al."Raman Spectroscopy in Colorectal Cancer Diagnostics: Comparison of PCA-LDA and PLS-DA Models".JOURNAL OF SPECTROSCOPY (2016):6. |
入库方式： OAI收割
来源：数学与系统科学研究院
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