Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition
文献类型：期刊论文
| 作者 | Fan, Wei1,2; Lam, Sin Man1; Xin, Jingxue2,3; Yang, Xiao1,2; Liu, Zhonghua1; Liu, Yuan1,2; Wang, Yong3 ; Shui, Guanghou1; Huang, Xun1,2
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| 刊名 | PLOS GENETICS
 |
| 出版日期 | 2017-03-01 |
| 卷号 | 13期号:3页码:21 |
| ISSN号 | 1553-7404 |
| DOI | 10.1371/journal.pgen.1006664 |
| 英文摘要 | The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid beta-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis. |
| 资助项目 | Strategic Priority Research Program oft he Chinese Academy of Sciences[XDB13030300/0400] ; National Basic Research Program of China[2013CB530606] ; National Basic Research Program of China[2016YFA0500100] ; National Natural Science Foundation of China[11422108] ; National Natural Science Foundation of China[31325018] ; National Natural Science Foundation of China[31571377] ; National Natural Science Foundation of China[31630019] |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000398043000040 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://ir.amss.ac.cn/handle/2S8OKBNM/25147]  |
| 专题 | 应用数学研究所 |
| 通讯作者 | Huang, Xun |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Wei,Lam, Sin Man,Xin, Jingxue,et al. Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition[J]. PLOS GENETICS,2017,13(3):21. |
| APA | Fan, Wei.,Lam, Sin Man.,Xin, Jingxue.,Yang, Xiao.,Liu, Zhonghua.,...&Huang, Xun.(2017).Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.PLOS GENETICS,13(3),21. |
| MLA | Fan, Wei,et al."Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition".PLOS GENETICS 13.3(2017):21. |
入库方式： OAI收割
来源：数学与系统科学研究院
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