N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search
文献类型：期刊论文
| 作者 | An, Zhiwu1,2; Shu, Qingbo2,3; Lv, Hao1,2,4; Shu, Lian2,3; Wang, Jifeng3; Yang, Fuquan2,3; Fu, Yan1,2
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| 刊名 | BIOMED RESEARCH INTERNATIONAL
 |
| 出版日期 | 2018 |
| 页码 | 11 |
| ISSN号 | 2314-6133 |
| DOI | 10.1155/2018/1564136 |
| 英文摘要 | Confident characterization of intact glycopeptides is a challenging task in mass spectrometry-based glycoproteomics due to microheterogeneity of glycosylation, complexity of glycans, and insufficient fragmentation of peptide bones. Open mass spectral library search is a promising computational approach to peptide identification, but its potential in the identification of glycopeptides has not been fully explored. Here we present pMatchGlyco, a new spectral library search tool for intact N-linked glycopeptide identification using high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) data. In pMatchGlyco, (1) MS/MS spectra of deglycopeptides are used to create spectral library, (2) MS/MS spectra of glycopeptides are matched to the spectra in library in an open (precursor tolerant) manner and the glycans are inferred, and (3) a false discovery rate is estimated for top-scored matches above a threshold. The efficiency and reliability of pMatchGlyco were demonstrated on a data set of mixture sample of six standard glycoproteins and a complex glycoprotein data set generated from human cancer cell line OVCAR3. |
| 资助项目 | International S&T Cooperation Program of China[2014DFB30010] ; National Key R & D Program of China[SQ2018YFA050014] ; Strategic Priority Research Programs of the Chinese Academy of Sciences[XDB13040600] ; Strategic Priority Research Programs of the Chinese Academy of Sciences[XDA12030202] ; National Key Basic Research Program of China[2015CB554406] ; National Natural Science Foundation of China[31670185] ; National Natural Science Foundation of China[91640112] ; NCMIS CAS |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000442867000001 |
| 出版者 | HINDAWI LTD |
| 源URL | [http://ir.amss.ac.cn/handle/2S8OKBNM/31101]  |
| 专题 | 应用数学研究所 |
| 通讯作者 | Yang, Fuquan; Fu, Yan |
| 作者单位 | 1.Chinese Acad Sci, Acad Math & Syst Sci, Natl Ctr Math & Interdisciplinary Sci, Key Lab Random Complex Struct & Data Sci, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Comp Network Informat Ctr, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Zhiwu,Shu, Qingbo,Lv, Hao,et al. N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search[J]. BIOMED RESEARCH INTERNATIONAL,2018:11. |
| APA | An, Zhiwu.,Shu, Qingbo.,Lv, Hao.,Shu, Lian.,Wang, Jifeng.,...&Fu, Yan.(2018).N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search.BIOMED RESEARCH INTERNATIONAL,11. |
| MLA | An, Zhiwu,et al."N-Linked Glycopeptide Identification Based on Open Mass Spectral Library Search".BIOMED RESEARCH INTERNATIONAL (2018):11. |
入库方式： OAI收割
来源：数学与系统科学研究院
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