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A novel approach to measure all rate constants in the simplest enzyme kinetics model

文献类型：期刊论文

作者Li, Banghe1,2; Li, Bo1,2,3; Shen, Yuefeng1,2,3
刊名JOURNAL OF MATHEMATICAL CHEMISTRY
出版日期2009-06-01
卷号46期号:1页码:290-301
关键词Michaelis constant Catalytic constant Michaelis-Menten equation
ISSN号0259-9791
DOI10.1007/s10910-008-9460-x
英文摘要Enzymes play vital roles in life processes. Almost all biochemical reactions are mediated by enzymes. The rate constants of enzyme kinetics are the most important parameters for the reactions catalyzed by enzymes. In 1902, Adrian Brown proposed a simple single-substrate-single-product model which contains only three rate constants k (1), k (-1) and k (2). So far, biologists can measure the Michaelis constant K (M) and the catalytic constant k (cat) , which actually is equal to k (2), according to Michaelis-Menten equation. Using temperature jump method or transient state kinetics, k (1), k (-1) and k (2) can be determined. However, these methods are complicated. In this article, we design a novel simple method that could determine the rate constants k (1) and k (-1) based on knowing k (cat) and K (M) . Our numerical experiments show that the three rate constants can be calculated rather precisely. Hence, we believe that biochemists could design experiments to measure the rate constants based on our method.
资助项目National Natural Science Foundation of China (NSFC)[10771206] ; 973 project[2004CB318000]
WOS研究方向Chemistry ; Mathematics
语种英语
WOS记录号WOS:000264886100018
出版者SPRINGER
源URL[http://ir.amss.ac.cn/handle/2S8OKBNM/7421]  
专题系统科学研究所
通讯作者Li, Banghe
作者单位1.Chinese Acad Sci, Ctr Bioinformat, Acad Math & Syst Sci, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Key Lab Math Mechanizat, Acad Math & Syst Sci, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Li, Banghe,Li, Bo,Shen, Yuefeng. A novel approach to measure all rate constants in the simplest enzyme kinetics model[J]. JOURNAL OF MATHEMATICAL CHEMISTRY,2009,46(1):290-301.
APA Li, Banghe,Li, Bo,&Shen, Yuefeng.(2009).A novel approach to measure all rate constants in the simplest enzyme kinetics model.JOURNAL OF MATHEMATICAL CHEMISTRY,46(1),290-301.
MLA Li, Banghe,et al."A novel approach to measure all rate constants in the simplest enzyme kinetics model".JOURNAL OF MATHEMATICAL CHEMISTRY 46.1(2009):290-301.

入库方式： OAI收割

来源：数学与系统科学研究院

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