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Src activation decouples cell division orientation from cell geometry in mammalian cells

文献类型：期刊论文

作者Sun, Xiaoyan1,2,3,4; Qi, Hongsheng5; Zhang, Xiuzhen1,2,3; Li, Li1,2,3,6; Zhang, Jiaping1,2,3; Zeng, Qunli1,2,3; Laszlo, George S.7; Wei, Bo8; Li, Tianhong9; Jiang, Jianxin10
刊名BIOMATERIALS
出版日期2018-07-01
卷号170页码:82-94
ISSN号0142-9612
关键词Cell division orientation Src Cell geometry Mitosis Cell polarity
DOI10.1016/j.biomaterials.2018.03.052
英文摘要Orientation of cell division plane plays a crucial role in morphogenesis and regeneration. Misoriented cell division underlies many important diseases, such as cancer. Studies with Drosophila and C. elegance models show that Src, a proto-oncogene tyrosine-protein kinase, is a critical regulator of this aspect of mitosis. However, the role for Src in controlling cell division orientation in mammalian cells is not well understood. Using genetic and pharmacological approaches and two extracellular signals to orient cell division, we demonstrated a critical role for Src. Either knockout or pharmacological inhibition of Src would retain the fidelity of cell division orientation with the long-axis orientation of mother cells. Conversely, re-expression of Src would decouple cell division orientation from the pre-division orientation of the long axis of mother cells. Cell division orientation in human breast and gastric cancer tissues showed that the Src activation level correlated with the degree of mitotic spindle misorientation relative to the apical surface. Examination of proteins associated with cortical actin revealed that Src activation regulated the accumulation and local density of adhesion proteins on the sites of cell-matrix attachment. By analyzing division patterns in the cells with or without Src activation and through use of a mathematical model, we further support our findings and provide evidence for a previously unknown role for Src in regulating cell division orientation in relation to the pre-division geometry of mother cells, which may contribute to the misoriented cell division. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目NIH[1R01EY019101] ; AFOSR[FA9550-16-1-0052] ; Research to Prevent Blindness ; NSFC[30901564] ; NSFC[81101883] ; NSFC[81721092] ; NSFC[81372067] ; NSFC[81230041] ; NSFC[81121004] ; NSFC[81421064] ; National Basic Science and Development Programme (973 Programme)[2012CB518105] ; Beijing Novel Program[2008B53] ; Beijing Novel Program[2009A38] ; National Key R&D Program of China[2017YFC1103304] ; National Key R&D Program of China[2016YFA0101000] ; National Key R&D Program of China[2016YFA0101002]
WOS研究方向Engineering ; Materials Science
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000432903300008
源URL[http://ir.amss.ac.cn/handle/2S8OKBNM/30450]  
专题系统科学研究所
通讯作者Fu, Xiaobing; Zhao, Min
作者单位1.Univ Calif Davis, Inst Regenerat Cures, Davis, CA 95616 USA
2.Univ Calif Davis, Dept Dermatol, Davis, CA 95616 USA
3.Univ Calif Davis, Dept Ophthalmol, Davis, CA 95616 USA
4.Chinese Peoples Liberat Army Gen Hosp, Postgrad Med Sch, Inst Basic Med Sci, Trauma Ctr,Wound Healing & Cell Biol Lab, 28 Fu Xing Rd, Beijing 100853, Peoples R China
5.Chinese Acad Sci, Acad Math & Syst Sci, Key Lab Syst & Control, 55 Zhongguancun East Rd, Beijing 100190, Peoples R China
6.Third Mil Med Univ, Doping Hosp, Dept Resp Dis, Chongqing 400042, Peoples R China
7.Fred Hutchinson Canc Res Ctr, Clin Res Div, 1100 Fairview Ave, Seattle, WA USA
8.Chinese Peoples Liberat Army Gen Hosp, Dept Gen Surg, 28 Fu Xing Rd, Beijing 100853, Peoples R China
9.Univ Calif Davis, Comprehens Canc Ctr, Div Hematol Oncol, 4501 X St 3016, Sacramento, CA 95817 USA
10.Third Mil Med Univ, Inst Surg, Doping Hosp, State Key Lab Trauma Burns & Combined Injury Res, Chongqing 400042, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Xiaoyan,Qi, Hongsheng,Zhang, Xiuzhen,et al. Src activation decouples cell division orientation from cell geometry in mammalian cells[J]. BIOMATERIALS,2018,170:82-94.
APA Sun, Xiaoyan.,Qi, Hongsheng.,Zhang, Xiuzhen.,Li, Li.,Zhang, Jiaping.,...&Zhao, Min.(2018).Src activation decouples cell division orientation from cell geometry in mammalian cells.BIOMATERIALS,170,82-94.
MLA Sun, Xiaoyan,et al."Src activation decouples cell division orientation from cell geometry in mammalian cells".BIOMATERIALS 170(2018):82-94.

入库方式： OAI收割

来源：数学与系统科学研究院

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