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An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla

文献类型：期刊论文

作者Xu, Wenyan1,2,3; Zhu, Hongtao2; Tan, Ninghua2; Tang, Jun2; Zhang, Yingjun2; Cerny, Ron L.1; Du, Liangcheng1
刊名PLANT CELL TISSUE AND ORGAN CULTURE
出版日期2012
卷号108期号:1页码:137-145
关键词Heterophyllin b Plant Cyclopeptide Biosynthesis Pseudostellaria Heterophylla Traditional Chinese Medicine
ISSN号0167-6857
DOI10.1007/s11240-011-0022-8
通讯作者Du, LC (reprint author), Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA, nhtan@mail.kib.ac.cn ; ldu@unlserve.unl.edu
文献子类Article
英文摘要Plant cyclopeptides are a large group of small molecule metabolites found in a wide variety of plants, including traditional Chinese medicinal plants. Many of the cyclopeptides have highly unusual structures and potent biological activities. However, the majority of the cyclopeptides have not been studied for their biosynthetic mechanisms. In this study, we have established a culture system for the biosynthetic study of heterophyllin B (HB), a cyclopeptide produced by the medicinal plant Pseudostellaria heterophylla. We first developed a shoot culture of P. heterophylla that produced HB consistently under laboratory conditions. Using 14 C-labeled proline as tracer, we showed that labeled HB was produced by the cultured shoots, indicating that this system has de novo biosynthetic activity. Next, we chemically synthesized HB's linear peptide precursor (LHB) and the N-acetyl cysteamine thioester of LHB (LHB-SNAC). When LHB-SNAC was incubated with total cell free extracts of the cultured shoots, a small amount of cyclized product (HB), in addition to the hydrolyzed product (LHB), was produced. The in vivo and in vitro results demonstrate the presence of an HB biosynthetic system, which provides insight into the molecular mechanism for plant cyclopeptide biosynthesis.
学科主题Biotechnology & Applied Microbiology ; Plant Sciences
WOS关键词PEPTIDE SYNTHETASES ; MICROCIN B17 ; RECONSTITUTION ; CYCLIZATION ; CYCLOTIDES ; CATALYZES ; PROTEINS
WOS研究方向Biotechnology & Applied Microbiology ; Plant Sciences
语种英语
WOS记录号WOS:000299329200015
资助机构Nebraska Research Council; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences; China Scholarship Council; NIH[RR015468-01]; NSFC[30725048]
公开日期2012-06-07
源URL[http://ir.kib.ac.cn:8080/handle/151853/10447]  
专题昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA
2.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Wenyan,Zhu, Hongtao,Tan, Ninghua,et al. An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2012,108(1):137-145.
APA Xu, Wenyan.,Zhu, Hongtao.,Tan, Ninghua.,Tang, Jun.,Zhang, Yingjun.,...&Du, Liangcheng.(2012).An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla.PLANT CELL TISSUE AND ORGAN CULTURE,108(1),137-145.
MLA Xu, Wenyan,et al."An in vitro system to study cyclopeptide heterophyllin B biosynthesis in the medicinal plant Pseudostellaria heterophylla".PLANT CELL TISSUE AND ORGAN CULTURE 108.1(2012):137-145.

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来源：昆明植物研究所

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