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  4. 植物化学与西部植物资源持续利用国家重点实验室
Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris

文献类型：期刊论文

作者Hou, Yuanyuan1,2; Cao, Xuelin3; Wang, Liqiang1,2; Cheng, Binfeng1,2; Dong, Linyi3; Luo, Xiaodong4; Bai, Gang1,2; Gao, Wenyuan3
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2012-11-01
卷号908页码:98-104
关键词Dual-bioactivity-integrated Fingerprinting Constituent Identification Nuclear Factor-kappa b Inhibitor Beta(2) Adrenergic Receptor Agonist Ultra Performance Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry
ISSN号1570-0232
DOI10.1016/j.jchromb.2012.10.004
通讯作者Bai, G (reprint author), Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.,gangbai@nankai.edu.cn ; pharmgao@tju.edu.cn
文献子类Article
英文摘要Traditional Chinese medicines (TCMs) are generally considered complementary or alternative remedies in most Western countries. The constituents of TCMs are hard to define, and their efficacy is difficult to appraise. Thus, the development of suitable methods for evaluating the relationship between bioactivity and the chemical makeup of complex TCM mixtures remains a great challenge. In the present work, the bioactivity-integrated fingerprints of alkaloidal leaf extracts of Alstonia scholaris, a folk medicinal herb for chronic respiratory diseases, were established by ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF). This method was coupled with two dual-luciferase reporter assay systems to show nuclear factor-kappa B (NF-kappa B) inhibition and beta(2) adrenergic receptor (beta(2)AR) activation. Using UPLC-Q/TOF, 18 potential candidates were identified according to unique mass spectrometric fragmentation. After in vitro biological evaluation, several indole alkaloids with anti-inflammatory and anti-asthmatic properties were found, including akuammidine, (E)-alstoscholarine, and (Z)-alstoscholarine. Compared with conventional fingerprints, the microfractionation based bioactivity-integrated fingerprints that contain both chemical and bioactivity details offer a more comprehensive understanding of the chemical makeup of plant materials. This strategy clearly demonstrated that dual bioactivity-integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines. (C) 2012 Elsevier B.V. All rights reserved.
学科主题Biochemical Research Methods ; Chemistry, Analytical
WOS关键词TRADITIONAL CHINESE MEDICINE ; FDA DRUG APPROVALS ; INFLAMMATORY DISEASES ; MULTITARGET DRUGS ; NATURAL-PRODUCTS ; TARGET ; THERAPEUTICS ; DISCOVERY ; SYSTEMS ; ASTHMA
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000311195100014
资助机构National Natural Science Foundation of China [81173638, 81001682]; Fundamental Research Funds for the Central Universities [65011111]
公开日期2013-01-22
源URL[http://ir.kib.ac.cn:8080/handle/151853/15922]  
专题昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300071, Peoples R China
3.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan Province, Peoples R China
推荐引用方式
GB/T 7714		 Hou, Yuanyuan,Cao, Xuelin,Wang, Liqiang,et al. Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2012,908:98-104.
APA Hou, Yuanyuan.,Cao, Xuelin.,Wang, Liqiang.,Cheng, Binfeng.,Dong, Linyi.,...&Gao, Wenyuan.(2012).Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,908,98-104.
MLA Hou, Yuanyuan,et al."Microfractionation bioactivity-based ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the identification of nuclear factor-kappa B inhibitors and beta(2) adrenergic receptor agonists in an alkaloidal extract of the folk herb Alstonia scholaris".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 908(2012):98-104.

入库方式： OAI收割

来源：昆明植物研究所

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