Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl
文献类型：期刊论文
| 作者 | Wang, Hao-Xin; Yang, Tao; Zeng, Ying ; Hu, Zhong
|
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 26期号:3页码:253-259 |
| 关键词 | Armillaria Mellea Gastrodia Antifungal Protein Gastrodia Elata Gastrodianins Mannose-binding Protein Orchid |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-006-0226-2 |
| 文献子类 | Article |
| 英文摘要 | Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiological functions in the plant are rarely understood. Aspects of expression of gastrodianins in growth and development of the plant will be helpful to dissect their functional roles. Two types of cDNA clones with complete cDNA sequences matching the known gastrodianins were obtained from G. elata Bl.f.glauca S.chow (Orchidaceae) and designated gastrodianin-4A (ga4A) and gastrodianin-4B (ga4B), respectively. But only one isoform was found to be expressed in all different parts of a single plant. Based on the RNA gel blot analysis, gastrodianins were much more abundantly expressed in the fully opened flowers than the underground corms where an enhanced expression was found in the out layers of secondary corms. By RNA in situ hybridization gastrodianin transcripts were distinctly detected in the cortical cells and vascular cells of corms. Strong transcript accumulations were observed in two to eight layers of cortical cells in secondary corms. From its peripheral tissue expression pattern and level in corms and flowers, the gastrodianin may account for a possible defense against phytopathogens or insects. |
| 学科主题 | Plant Sciences |
| WOS关键词 | MANNOSE-BINDING PROTEINS ; SPECIES LISTERA-OVATA ; EPIPACTIS-HELLEBORINE ; ANTIFUNGAL PROTEIN ; MOLECULAR-CLONING ; LECTINS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000244200500001 |
| 公开日期 | 2011-11-24 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/2111]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hao-Xin,Yang, Tao,Zeng, Ying,et al. Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl[J]. PLANT CELL REPORTS,2007,26(3):253-259. |
| APA | Wang, Hao-Xin,Yang, Tao,Zeng, Ying,&Hu, Zhong.(2007).Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl.PLANT CELL REPORTS,26(3),253-259. |
| MLA | Wang, Hao-Xin,et al."Expression analysis of the gastrodianin gene ga4B in an achlorophyllous plant Gastrodia elata Bl".PLANT CELL REPORTS 26.3(2007):253-259. |
入库方式： OAI收割
来源：昆明植物研究所
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