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Isolation and characterization of cytotoxic cyclotides from Viola tricolor

文献类型：期刊论文

作者Tang, Jun1,3; Wang, Conan K.2; Pan, Xulin1; Yan, He1,3; Zeng, Guangzhi1; Xu, Wenyan1,3; He, Wenjun1,3; Daly, Norelle L.2; Craik, David J.2; Tan, Ninghua1
刊名PEPTIDES
出版日期2010-08-01
卷号31期号:8页码:1434-1440
关键词Cyclotides Viola Tricolor Cytotoxic Activity Vitri B-f Varv Hm Varv He
ISSN号0196-9781
DOI10.1016/j.peptides.2010.05.004
文献子类Article
英文摘要Many plants of the Violaceae plant family have been used in traditional remedies, and these plants often contain cyclotides, a particular type of plant cyclopeptide that is distinguished by a cyclic cystine knot motif. In general, bioactive plant cyclopeptides are interesting candidates for drug development. In the current study, a suite of 14 cyclotides, which includes seven novel cyclotides [vitri B, C, D, E, F, vary Hm, and He], together with seven known cyclotides [vary A, D, E, F, H, vitri A, and cycloviolacin O2], was isolated from Viola tricolor, a common flower. A chromatography-based method was used to isolate the cyclotides, which were characterized using tandem mass spectrometry and NMR spectroscopy. Several of the cyclotides showed cytotoxic activities against five cancer cell lines, U251, MDA-MB-231, A549, DU145, and BEL-7402. Three cyclotides, vitri A, vitri F, and cycloviolacin O2, were the most cytotoxic. The cytotoxic activity of the cyclotides did not correlate well with their hemolytic activity, indicating that different interactions, most likely with membranes, are involved for cytotoxic and hemolytic activities. Homology modeling of the structures was used in deriving structure-activity relationships. (C) 2010 Elsevier Inc. All rights reserved.
学科主题Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
WOS关键词CYCLIC CYSTINE KNOT ; TLC CHEMICAL METHOD ; KALATA B1 ; MACROCYCLIC PEPTIDES ; CYCLOVIOLACIN O2 ; DRUG DESIGN ; SCANNING MUTAGENESIS ; OLDENLANDIA-AFFINIS ; SEQUENCE VARIATION ; CIRCULAR PROTEINS
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000280663600003
资助机构NSFC ; ARC ; National Natural Science Foundation of China [30725048]; National Basic Research Program of China [2009 CB522300]; Science and Technology Department of Yunnan Province [2008OC011]; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences
公开日期2012-03-21
源URL[http://ir.kib.ac.cn:8080/handle/151853/4868]  
专题昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources W China, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Jun,Wang, Conan K.,Pan, Xulin,et al. Isolation and characterization of cytotoxic cyclotides from Viola tricolor[J]. PEPTIDES,2010,31(8):1434-1440.
APA Tang, Jun.,Wang, Conan K..,Pan, Xulin.,Yan, He.,Zeng, Guangzhi.,...&Tan, Ninghua.(2010).Isolation and characterization of cytotoxic cyclotides from Viola tricolor.PEPTIDES,31(8),1434-1440.
MLA Tang, Jun,et al."Isolation and characterization of cytotoxic cyclotides from Viola tricolor".PEPTIDES 31.8(2010):1434-1440.

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来源：昆明植物研究所

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