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PanB is involved in nicotine metabolism in Pseudomonas putida

文献类型：期刊论文

作者Wei, Hailei1,2; Lei, Liping3; Liu, Shuo4; Xia, Zhenyuan3; Liu, Xingzhong1; Liu, Peigui2
刊名INTERNATIONAL BIODETERIORATION & BIODEGRADATION
出版日期2009-12-01
卷号63期号:8页码:988-992
关键词Biodegradation Nicotine Pseudomonas Putida Ketopantoate Hydroxymethyltransferase
ISSN号0964-8305
DOI10.1016/j.ibiod.2009.02.010
通讯作者liuxz@im.ac.cn ; pgliu@kib.ac.cn
文献子类Article
英文摘要A nicotine-sensitive mutant was generated from the nicotine-degrading bacterium, Pseudomonas putida strain J5, by mini-Tn5 transposon mutagenesis. This mutant was unable to grow with nicotine as the sole carbon source but could grow with glucose. Sequence analysis showed that the Tn5 transposon inserted at the site of the ketopantoate hydroxymethyltransferase gene (panB), which had 54% identity to PanB in Escherichia coli K-12. In-frame deletion of the panB gene abolished the nicotine-degrading ability of strain J5, while complementation with panB from P putida J5 and E. coli K-12 restored the degrading activity of the mutant to the wild-type level. These results suggest that ketopantoate hydroxymethyltransferase is a crucial enzyme in nicotine metabolism in P putida J5. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题Biotechnology & Applied Microbiology ; Environmental Sciences
WOS关键词ARTHROBACTER-NICOTINOVORANS ; ESCHERICHIA-COLI ; KETOPANTOATE HYDROXYMETHYLTRANSFERASE ; PANTOTHENATE BIOSYNTHESIS ; STRAIN S16 ; DEGRADATION ; GENE ; PLASMID ; CLONING ; CATABOLISM
WOS研究方向Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000272218800004
资助机构National Natural Science Foundation of China [30460007, 30760011]; Science and Technology Program of the Yunnan Tobacco Co. Ltd [04A17]
公开日期2011-12-20
源URL[http://ir.kib.ac.cn:8080/handle/151853/2984]  
专题昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Inst Microbiol, Key Lab Systemat Mycol & Lichenol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China
3.Yunnan Tobacco Res Inst, Yuxi, Peoples R China
4.Beijing Jiaotong Univ, Affiliated High Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Wei, Hailei,Lei, Liping,Liu, Shuo,et al. PanB is involved in nicotine metabolism in Pseudomonas putida[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2009,63(8):988-992.
APA Wei, Hailei,Lei, Liping,Liu, Shuo,Xia, Zhenyuan,Liu, Xingzhong,&Liu, Peigui.(2009).PanB is involved in nicotine metabolism in Pseudomonas putida.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,63(8),988-992.
MLA Wei, Hailei,et al."PanB is involved in nicotine metabolism in Pseudomonas putida".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 63.8(2009):988-992.

入库方式： OAI收割

来源：昆明植物研究所

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