Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting
文献类型：期刊论文
| 作者 | Yang, Yang1 ; Pan, Yuezhi1; Gong, Xun1; Fan, Moutian2
|
| 刊名 | GENETIC RESOURCES AND CROP EVOLUTION
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 57期号:8页码:1239-1248 |
| 关键词 | Citrus Hongheensis Conservation Differentiation Genetic Diversity Issr Fingerprinting Rutaceae |
| ISSN号 | 0925-9864 |
| DOI | 10.1007/s10722-010-9571-7 |
| 通讯作者 | gongxun@mail.kib.ac.cn |
| 文献子类 | Article |
| 英文摘要 | Citrus hongheensis is a critically endangered species endemic to the Honghe river region in southeastern Yunnan, China. Its genetic diversity and differentiation were investigated using Inter-Simple Sequence Repeat (ISSR) markers. One hundred primers were screened, and a total of 245 loci were amplified from seven natural populations by 13 informative and reliable primers. Of these 245 ISSR loci, 233 were polymorphic and the detected variations revealed a relatively high level of intraspecific genetic diversity. At the population level, the mean percentage of polymorphic loci (PPB) was 36.50%, while the average expected heterozygosity (He) and Shannon diversity index (Ho) were 0.1327 and 0.1972, respectively. At the species level (across all populations), PPB was 95.10%, while He and Ho were 0.3520 and 0.5195, respectively. A high Gst value (0.6247) indicated that there is significant differentiation among populations, which was confirmed by AMOVA analysis (I broken vertical bar st = 0.6420). Pairwise genetic identity (I) values among populations ranged from 0.6341 to 0.7675, with a mean of 0.7008. We propose that the high level of genetic differentiation may be the result of habitat fragmentation and limited gene flow (Nm = 0.1502). For effective in situ conservation and population restoration of C. hongheensis it will be important to maintain historical processes, including high outbreeding rates, sufficient gene flow, and large effective population sizes. |
| 学科主题 | Agronomy ; Plant Sciences |
| WOS关键词 | DIVERSITY ; CONSERVATION ; POPULATIONS ; PLANTS ; FLOW ; CONSEQUENCES ; SIZE ; RARE |
| WOS研究方向 | Agriculture ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000284777400012 |
| 资助机构 | National Basic Research Program of China [2007CB411600]; National Natural Science Foundation of China [30670210] |
| 公开日期 | 2011-12-20 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/3180]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China 2.Yunnan Agr Univ, Kunming 650201, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yang,Pan, Yuezhi,Gong, Xun,et al. Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting[J]. GENETIC RESOURCES AND CROP EVOLUTION,2010,57(8):1239-1248. |
| APA | Yang, Yang,Pan, Yuezhi,Gong, Xun,&Fan, Moutian.(2010).Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting.GENETIC RESOURCES AND CROP EVOLUTION,57(8),1239-1248. |
| MLA | Yang, Yang,et al."Genetic variation in the endangered Rutaceae species Citrus hongheensis based on ISSR fingerprinting".GENETIC RESOURCES AND CROP EVOLUTION 57.8(2010):1239-1248. |
入库方式： OAI收割
来源：昆明植物研究所
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