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Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)

文献类型：期刊论文

作者Chen, Shaotian1; Wu, Xiayu2; Ji, Yunheng1; Yang, Junbo3
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
出版日期2011-11-01
卷号12期号:11页码:7818-7823
关键词Pistacia Weinmannifolia Microsatellites Anacardiaceae Genetic Structure Polymorphism
ISSN号1661-6596
DOI10.3390/ijms12117818
通讯作者chenst@kib.ac ; xiayu98wu@yahoo.com.cn ; jiyh@mail.kib.ac.cn ; jbyang@mail.kib.ac.cn
文献子类Article
英文摘要Fourteen polymorphic microsatellite loci were isolated from the genomic DNA of Pistacia weinmannifolia, using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) method, and screened on 12 individuals from each of two wild populations. The 14 polymorphic loci had an average of 4.1 alleles per locus varying from 1 to 9. The observed (H-o) and expected (He) heterozygosities across the two populations ranged from 0.000 to 0.933 and from 0.000 to 0.906, respectively. Tests for departure from Hardy-Weinberg equilibrium (HWE) and genotypic linkage disequilibrium (LD) were conducted for each of the two populations separately. It was found that no locus significantly deviated from HWE proportions and no significant LD was detected between loci (p < 0.001). In the test of cross-species utility, we successfully amplified nine (64.2%) of 14 loci in P. chinensis and four (28.6%) in P. mexicana. The relatively high level of polymorphism for these markers will facilitate further studies of gene flow, population structure and evolutionary history of P. weinmannifolia and its congeners.
学科主题Chemistry, Multidisciplinary
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000297696100037
资助机构National Natural Science Foundation of China[30770137, 31070198]; Chinese Academy of Sciences[2009-LSF-GBOWS-01]; GBOWS
公开日期2012-03-16
源URL[http://ir.kib.ac.cn:8080/handle/151853/3898]  
专题昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Peoples R China
2.Yunnan Normal Univ, Sch Life Sci, Kunming 650092, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species SW China, Kunming 650204, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Shaotian,Wu, Xiayu,Ji, Yunheng,et al. Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(11):7818-7823.
APA Chen, Shaotian,Wu, Xiayu,Ji, Yunheng,&Yang, Junbo.(2011).Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(11),7818-7823.
MLA Chen, Shaotian,et al."Isolation and Characterization of Microsatellite Loci in Pistacia weinmannifolia (Anacardiaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.11(2011):7818-7823.

入库方式： OAI收割

来源：昆明植物研究所

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