Structural analysis of a homogeneous polysaccharide from Achatina fulica
文献类型:期刊论文
作者 | Liu,Jie![]() ![]() |
刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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出版日期 | 2017 |
卷号 | 98页码:786-792 |
关键词 | Anticoagulant Activities Acharan Sulfate Sea-cucumber Glycosaminoglycan Chromatography Spectroscopy Derivatives Snail Polysaccharide Chemical Structure Nmr |
ISSN号 | 0141-8130 |
DOI | 10.1016/j.ijbiomac.2017.01.149 |
英文摘要 | Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structureof the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy (H-1,C-13, COSY, TOCSY, NOESY, HSQC and HMBC). Chemical composition analysis indicated that AF-1 is composed of glucose (Glc) and its average molecular weight is 1710 kDa. Structural analysis suggested that AF-1 is mainly consisted of a linear repeating backbone of (1 -> 4) linked alpha-D-Glc p residues with one branch, a-D-Glc p, attached to the main chain by (1 -> 6) glycosidic bonds at every five main-chain units. Further studies on biological activities of the polysaccharide are currently in progress. (C) 2017 Elsevier B.V. All rights reserved. |
学科主题 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
语种 | 英语 |
源URL | [http://ir.kib.ac.cn/handle/151853/54953] ![]() |
专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
推荐引用方式 GB/T 7714 | Liu,Jie,Shang,Feineng,Yang,Zengming,et al. Structural analysis of a homogeneous polysaccharide from Achatina fulica[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,98:786-792. |
APA | Liu,Jie,Shang,Feineng,Yang,Zengming,Wu,Mingyi,&Zhao,Jinhua.(2017).Structural analysis of a homogeneous polysaccharide from Achatina fulica.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,98,786-792. |
MLA | Liu,Jie,et al."Structural analysis of a homogeneous polysaccharide from Achatina fulica".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 98(2017):786-792. |
入库方式: OAI收割
来源:昆明植物研究所
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