A re-evaluation of dilution for eliminating PCR inhibition in soil DNA samples
文献类型：期刊论文
| 作者 | Wang,Hang; Qi,Jinfeng; Xiao,Derong; Wang,Zhibao; Tian,Kun |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 106页码:109-118 |
| 关键词 | Real-time Pcr Environmental-samples Extraction Sediment Quantification Optimization Assay Pcr Inhibition Dilution Dna Extraction Microbe Humic Acid |
| ISSN号 | 0038-0717 |
| DOI | 10.1016/j.soilbio.2016.12.011 |
| 英文摘要 | Quantitative real-time PCR (qPCR) analysis for accurate quantification of targeted microbial genes is compromised by the presence of co-extracted inhibitors from soil samples. Dilution of DNA extracts is a commonly-used method to reduce levels of inhibition. However, the applications of dilution method are mostly empirical, and need to be further elaborated. Here, we propose a dilution model to re-evaluate dilution as a method to eliminate qPCR inhibition. We found that DNA extracts without dilution or with a minor dilution (e.g., 10-fold) resulted in ciPCR inhibition for most of studied soils. However, excessive dilution (e.g., 200- or 400-fold) caused an overestimation of the quantified gene cdpy numbers. Only under a moderate dilution range could qPCR inhibition be efficiehtly eliminated, which has been well captured by our proposed dilution model. The pre-testing of qPCR inhibition for determining the appropriate dilution range for extracted DNA samples aids accurate quantification of nucleic acids in soils. (C) 2016 Elsevier Ltd. All rights reserved. |
| 学科主题 | Soil Science |
| 语种 | 英语 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/54961]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang,Hang,Qi,Jinfeng,Xiao,Derong,et al. A re-evaluation of dilution for eliminating PCR inhibition in soil DNA samples[J]. SOIL BIOLOGY & BIOCHEMISTRY,2017,106:109-118. |
| APA | Wang,Hang,Qi,Jinfeng,Xiao,Derong,Wang,Zhibao,&Tian,Kun.(2017).A re-evaluation of dilution for eliminating PCR inhibition in soil DNA samples.SOIL BIOLOGY & BIOCHEMISTRY,106,109-118. |
| MLA | Wang,Hang,et al."A re-evaluation of dilution for eliminating PCR inhibition in soil DNA samples".SOIL BIOLOGY & BIOCHEMISTRY 106(2017):109-118. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。