Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus
文献类型：期刊论文
| 作者 | Qin, Yujing1; Yuan, Qingxia1; Zhang, Yuexing1; Li, Jialu1; Zhu, Xinjiao1; Zhao, Lingling1; Wen, Jing2; Liu, Jikai1; Zhao, Longyan1 ; Zhao, Jinhua1,3
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2018-03-01 |
| 卷号 | 23期号:3页码:590 |
| 关键词 | Sea Cucumber Polysaccharide Enzyme-assisted Extraction Characterization Antioxidant Activity |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules23030590 |
| 英文摘要 | Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumber Phyllophorus proteus (PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 +/- 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 degrees C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98C1 and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted of N-acetyl-galactosamine (Ga1NAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, Ga1NAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides from Phyllophorus proteus may be novel agents having potential value for antioxidation. |
| 语种 | 英语 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/61254]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Hubei, Peoples R China 2.Lingnan Normal Univ, Coll Life Sci & Technol, Zhanjiang 524048, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Yujing,Yuan, Qingxia,Zhang, Yuexing,et al. Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus[J]. MOLECULES,2018,23(3):590. |
| APA | Qin, Yujing.,Yuan, Qingxia.,Zhang, Yuexing.,Li, Jialu.,Zhu, Xinjiao.,...&Zhao, Jinhua.(2018).Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus.MOLECULES,23(3),590. |
| MLA | Qin, Yujing,et al."Enzyme-Assisted Extraction Optimization, Characterization and Antioxidant Activity of Polysaccharides from Sea Cucumber Phyllophorus proteus".MOLECULES 23.3(2018):590. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。