Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro
文献类型：期刊论文
| 作者 | Wu, Xin1,2; Xie, Chuan-qi1,2; Zhu, Qiang-qiang1,2; Wang, Ming-yue1,2; Sun, Bin1,2; Huang, Yan-ping1,2; Shen, Chang1,2; An, Meng-Fei1,2; Zhao, Yun-li1,2,3; Wang, Xuan-jun1,4,5 |
| 刊名 | FOOD & NUTRITION RESEARCH
 |
| 出版日期 | 2018-10-08 |
| 卷号 | 62页码:11 |
| 关键词 | green tea aqueous extract osteoporosis ovariectomy receptor activator of the nuclear factor kappa B ligand osteoclast |
| ISSN号 | 1654-6628 |
| DOI | 10.29219/fnr.v62.1478 |
| 英文摘要 | Background: Green tea (Camelliasinensis [L.] Kuntze) belongs to the plant family Theaceae and is mainly distributed in East Asia, the Indian subcontinent and Southeast Asia. This plant has been proven to be beneficial to human health, and green tea is the second most consumed beverage in the world after water. However, until now, the effect of green tea aqueous extract (GTE) upon postmenopausal osteoporosis has remained unclear. In this study, we investigated the therapeutic effects of GTE on estrogen deficiency-induced osteoporosis and explored the possible mechanisms in vivo and in vitro. Materials and methods: Ovariectomized (OVX) female rats were orally administered with GTE at doses of 60, 120, and 370 mg kg(-1) for 13 consecutive weeks. The biochemical parameters, bone gla protein, alkaline phosphatase, acid phosphatase, estrogen, interleukin-1 beta, and interleukin-6 in blood samples were detected, and histological change in bones was analyzed by hematoxylin and eosin staining. Meanwhile, the mechanisms of GTE on osteoclast formation were explored in RAW 264.7 cells induced by receptor activation of the nuclear factor kappa B ligand (RANKL). Results: The results showed that GTE could increase bone mass and inhibit trabecular bone loss in OVX rats. Furthermore, real-time quantitative reverse transcription polymerase chain reaction analysis from in vitro experiments also showed that GTE reduced the mRNA expression of osteoclast-associated genes such as cathepsin K (cath-K), c-Fos, matrix metalloproteinase 9, nuclear factor of activated T cells cytoplasmic 1 (NFATcl) and tartrate-resistant acid phosphatase. In addition, GTE caused a reduction in the protein levels of NFATcl, c-Fos, c-src and cath-K. Conclusion: Evidence from both animal models and in vitro experiments suggested that GTE might effectively ameliorate the symptoms of osteoporosis in OVX rats and inhibit RANKL-induced osteoclast-specific gene and protein expression. |
| 资助项目 | Scholarship for Talent Academic Leader of Yunnan Province[2017HA015] ; Scholarship for Young Academic and Technological Leaders of Yunnan Province[2015HB037] ; Science and Technology Major Project of Yunnan Province[2017ZF003] |
| WOS研究方向 | Food Science & Technology ; Nutrition & Dietetics |
| 语种 | 英语 |
| WOS记录号 | WOS:000446665100001 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 资助机构 | Scholarship for Talent Academic Leader of Yunnan Province ; Scholarship for Young Academic and Technological Leaders of Yunnan Province ; Science and Technology Major Project of Yunnan Province |
| 源URL | [http://ir.kib.ac.cn/handle/151853/62775]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Zhao, Yun-li; Wang, Xuan-jun; Sheng, Jun |
| 作者单位 | 1.Yunnan Agr Univ, Key Lab Pu Erh Tea Sci, Minist Educ, Kunming, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 4.Yunnan Agr Univ, Coll Sci, Kunming, Yunnan, Peoples R China 5.State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xin,Xie, Chuan-qi,Zhu, Qiang-qiang,et al. Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro[J]. FOOD & NUTRITION RESEARCH,2018,62:11. |
| APA | Wu, Xin.,Xie, Chuan-qi.,Zhu, Qiang-qiang.,Wang, Ming-yue.,Sun, Bin.,...&Sheng, Jun.(2018).Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro.FOOD & NUTRITION RESEARCH,62,11. |
| MLA | Wu, Xin,et al."Green tea (Camellia sinensis) aqueous extract alleviates postmenopausal osteoporosis in ovariectomized rats and prevents RANKL-induced osteoclastogenesis in vitro".FOOD & NUTRITION RESEARCH 62(2018):11. |
入库方式： OAI收割
来源：昆明植物研究所
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