Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds
文献类型：期刊论文
| 作者 | Yan, N; Hu, H ; Huang, JL; Xu, K; Wang, H; Zhou, ZK
|
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2006 |
| 卷号 | 84期号:1页码:113-117 |
| ISSN号 | 0167-6857 |
| 关键词 | Cypripedium Flavum Conservation Induction Of Multiple Shoots Micropropagation Root Formation |
| DOI | 10.1007/s11240-005-9001-2 |
| 通讯作者 | huhong@mail.kib.ac.cn |
| 文献子类 | Article |
| 英文摘要 | Cypripedium flavum, known as the rare lady's slipper orchid, is one of the endemics with a yellow flower in China. Due to its conservation and commercial requirement, establishment of an efficient method for micropropogation is urgently needed. Multiple shoots were obtained by placing seedlings from seeds of C. flavum on Harvais media supplemented with two cytokinins (BAP or KIN) used alone or in addition to different concentration of potato homogenate. The effect of BAP was better than that of KIN on shoot multiplication. The Havais media supplemented with BAP (2.22 mu M) and potato homogenate (20 g l(-1)) was the most effective, providing high shoot multiplication frequencies (95%) associated with a high number of shoots per explant (2.55 shoots/plant). For root formation, high rooting and survival were achieved using 1/2 Harvais media supplemented with 0.6 g l(-1) activated charcoals. High-level activated charcoal increased the number and the length of roots because the activated charcoal could absorb BAP in the media. This study demonstrated that C. flavum could be micropropagated by using multiple shoots of seedlings derived from mature seeds. |
| 学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS关键词 | PLANT-REGENERATION ; CALLUS ; GROWTH |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000236371200011 |
| 公开日期 | 2011-12-06 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/2429]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, N,Hu, H,Huang, JL,et al. Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2006,84(1):113-117. |
| APA | Yan, N,Hu, H,Huang, JL,Xu, K,Wang, H,&Zhou, ZK.(2006).Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds.PLANT CELL TISSUE AND ORGAN CULTURE,84(1),113-117. |
| MLA | Yan, N,et al."Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from mature seeds".PLANT CELL TISSUE AND ORGAN CULTURE 84.1(2006):113-117. |
入库方式： OAI收割
来源：昆明植物研究所
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