中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 昆明植物研究所
  3. 中国科学院昆明植物研究所
  4. 资源植物与生物技术所级重点实验室
In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)

文献类型：期刊论文

作者Long, Bo1,2; Niemiera, Alex X.3; Cheng, Zhi-ying1; Long, Chun-lin1,4
刊名PLANT CELL TISSUE AND ORGAN CULTURE
出版日期2010-05-01
卷号101期号:2页码:151-162
关键词Micropropagation Orchid Seed Germination Shoot Proliferation
ISSN号0167-6857
DOI10.1007/s11240-010-9672-1
通讯作者longbo@mail.kib.ac.cn ; niemiera@vt.edu ; long@mail.kib.ac.cn
文献子类Article
英文摘要The effects of seed maturity, media type, carbon source, and organic nutrient additives on seed germination, protocorm development, and plant growth of Paphiopedilum villosum var. densissimum Z. J. Liu et S. C. Chen were investigated. Micropropagation frequency was enhanced through the use of 200-day-old seed, Knudson C (KC) medium, and the presence of both glucose and coconut milk in the medium. The effects of various plant growth regulators on the frequency of shoot organogenesis in four Paphiopedilum species were also investigated. Explants of P. villosum var. densissimum and P. insigne (Lindl.) Stein incubated in the presence of 5 mg l(-1) 6-benzyladenine (BA) with 0.5 mg l(-1) alpha-naphthalene acetic acid (NAA) and 0.2 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, showed a twofold increase in the frequency of shoot organogenesis. For explants of P. bellatulum (Rchb. f.) Stein and P. armeniacum S. C. Chen et F. Y. Liu, the combination of 5.5 mg l(-1) BA with 0.5 mg l(-1) NAA and 4 mg l(-1) BA with 0.1 mg l(-1) NAA, respectively, resulted in the highest frequencies of shoot organogenesis.
学科主题Biotechnology & Applied Microbiology ; Plant Sciences
WOS关键词ASYMBIOTIC SEED-GERMINATION ; PLANT-REGENERATION ; SLIPPER ORCHID ; SHOOT MULTIPLICATION ; CULTURE ; CALLUS ; DELENATII ; INVITRO ; INDUCTION ; GROWTH
WOS研究方向Biotechnology & Applied Microbiology ; Plant Sciences
语种英语
WOS记录号WOS:000277204500005
资助机构The authors thank Chunyan Han, Guangwan Hu, and Yuan Liu for their kind help. This work was supported by the Natural Science Foundation of Yunnan (2005C0053 M), the Ministry of Science and Technology of China (2005DK21006) and the Ministry of Education of China (B08044 & MUC-985-3-3).
公开日期2011-12-06
源URL[http://ir.kib.ac.cn:8080/handle/151853/2431]  
专题昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Virginia Polytech & State Univ, Dept Hort, Blacksburg, VA 24061 USA
4.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714		 Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,et al. In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,101(2):151-162.
APA Long, Bo,Niemiera, Alex X.,Cheng, Zhi-ying,&Long, Chun-lin.(2010).In vitro propagation of four threatened Paphiopedilum species (Orchidaceae).PLANT CELL TISSUE AND ORGAN CULTURE,101(2),151-162.
MLA Long, Bo,et al."In vitro propagation of four threatened Paphiopedilum species (Orchidaceae)".PLANT CELL TISSUE AND ORGAN CULTURE 101.2(2010):151-162.

入库方式： OAI收割

来源：昆明植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。