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Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana

文献类型：期刊论文

作者Hu, Xiangyang1; Neill, Stevn J.2; Yang, Yongping1; Cai, Weiming3
刊名PLANTA
出版日期2009-05-01
卷号229期号:6页码:1201-1208
关键词Arabidopsis Aequorin Cytosolic Calcium Ions Hydrogen Peroxide Octadecanoid Pathway
ISSN号0032-0935
DOI10.1007/s00425-009-0909-3
通讯作者huxiangyang@mail.kib.ac.cn ; wmcai@sibs.ac.cn
文献子类Article
英文摘要Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.; Transgenic Arabidopsis thaliana plants expressing the protein aequorin were used to investigate the transient change in cytosolic calcium ions caused by stimulation through the fungal elicitor Pep-25. Our results show that the elicitor Pep-25 derived from Phytophthora sojae can induce H2O2 production and an increase in cytosolic calcium ions. The transcription of LOX, OPR3, PED, AOS, AOC genes and the protein accumulation of AOS were induced by Pep-25 treatment. Pep-25 also induced an accumulation of jasmonic acid (JA). Blocking the production of H2O2 and the increase of cytosolic calcium ions both suppressed the transcription of LOX, OPR3, PED, AOS, AOC genes, the accumulation of AOS, and the accumulation of JA. These results indicated that the production of H2O2 derived from the plasma-membrane NADPH oxidase and the subsequently increase of cytosolic calcium ions are both required for the activation of the octadecanoid pathway by Pep-25 treatment in A. thaliana.
学科主题Plant Sciences
WOS关键词PLANT-DISEASE RESISTANCE ; OXIDATIVE BURST ; DEFENSE RESPONSES ; HYDROGEN-PEROXIDE ; GENE-EXPRESSION ; JASMONIC ACID ; CA2+ FLUXES ; CELLS ; OXYGEN ; INDUCTION
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000265540100004
资助机构National Natural Science Foundation in China [30570157]; National Natural Science Foundation [30871704]
公开日期2011-12-06
源URL[http://ir.kib.ac.cn:8080/handle/151853/2651]  
专题昆明植物研究所_中国科学院青藏高原研究所昆明部
作者单位1.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Univ W England, Ctr Res Plant Sci, Bristol BS16 1QY, Avon, England
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 2000032, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Xiangyang,Neill, Stevn J.,Yang, Yongping,et al. Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana[J]. PLANTA,2009,229(6):1201-1208.
APA Hu, Xiangyang,Neill, Stevn J.,Yang, Yongping,&Cai, Weiming.(2009).Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana.PLANTA,229(6),1201-1208.
MLA Hu, Xiangyang,et al."Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana".PLANTA 229.6(2009):1201-1208.

入库方式： OAI收割

来源：昆明植物研究所

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