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Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity

文献类型：期刊论文

作者Wang, Lina1,2; Yang, Liming3; Yang, Fengjuan1,2; Li, Xiaogang1,2; Song, Yunpeng1,2; Wang, Xiufeng1,2; Hu, Xiangyang4
刊名JOURNAL OF PLANT PHYSIOLOGY
出版日期2010
卷号167期号:15页码:1298-1306
关键词Copper Hydrogen Peroxide Metallothionein Nitric Oxide Tomato
ISSN号0176-1617
DOI10.1016/j.jplph.2010.04.007
通讯作者xfwang@sdau.edu.cn ; huxiangyang@mail.kib.ac.cn
文献子类Article
英文摘要Both H2O2 and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to copper toxicity. We found that Cu2+ stress caused the rapid release of H2O2 and chlorotic leaves, and it stunted root growth and development. Cu treatment also caused an increase in NOS enzyme activity and NO release in roots and leaves. Application of the NO donor SNP efficiently alleviated the copper toxicity effect, as shown by increases in chlorophyll content and the biomass of fresh/dry leaves. SNP treatment also induced the transcription and increased activities of anti-oxidant enzymes, including catalase, peroxidase, superoxide dismutase and ascorbate peroxidase, and led to reduced H2O2 accumulation in the leaves. Special inhibitors or scavengers of NO synthesis diminished the ameliorating effect of NO on copper toxicity. NO application induced MT transcription and accumulation in leaves. Furthermore, the antisense-MT transgenic tomato was more sensitive to copper stress, and this effect could not be efficiently reversed by NO treatment. From these data, we propose that NO induces tomato tolerance to copper toxicity through antioxidant enzyme activity and metallothionein accumulation, and that metallothionein acts downstream of NO signaling. (C) 2010 Elsevier GmbH. All rights reserved.; Both H2O2 and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallothionein in tomato plant responses to copper toxicity. We found that Cu2+ stress caused the rapid release of H2O2 and chlorotic leaves, and it stunted root growth and development. Cu treatment also caused an increase in NOS enzyme activity and NO release in roots and leaves. Application of the NO donor SNP efficiently alleviated the copper toxicity effect, as shown by increases in chlorophyll content and the biomass of fresh/dry leaves. SNP treatment also induced the transcription and increased activities of anti-oxidant enzymes, including catalase, peroxidase, superoxide dismutase and ascorbate peroxidase, and led to reduced H2O2 accumulation in the leaves. Special inhibitors or scavengers of NO synthesis diminished the ameliorating effect of NO on copper toxicity. NO application induced MT transcription and accumulation in leaves. Furthermore, the antisense-MT transgenic tomato was more sensitive to copper stress, and this effect could not be efficiently reversed by NO treatment. From these data, we propose that NO induces tomato tolerance to copper toxicity through antioxidant enzyme activity and metallothionein accumulation, and that metallothionein acts downstream of NO signaling. (C) 2010 Elsevier GmbH. All rights reserved.
学科主题Plant Sciences
WOS关键词NITRIC-OXIDE FUNCTIONS ; OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; SUPEROXIDE-DISMUTASE ; ARABIDOPSIS-THALIANA ; DISEASE-RESISTANCE ; CUCUMBER PLANTS ; HEAVY-METALS ; RICE LEAVES ; ROOT-GROWTH
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000282911300011
资助机构National Science Foundation of China [30871704, 30971452, 30900871, 30871705, 30900983]; CAS ; Natural Science Foundation of Jiangshu Higher Education Institutions in China [09KJB210001]
公开日期2011-12-06
源URL[http://ir.kib.ac.cn:8080/handle/151853/2653]  
专题昆明植物研究所_中国科学院青藏高原研究所昆明部
作者单位1.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China
2.State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
3.Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China
4.Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Lina,Yang, Liming,Yang, Fengjuan,et al. Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity[J]. JOURNAL OF PLANT PHYSIOLOGY,2010,167(15):1298-1306.
APA Wang, Lina.,Yang, Liming.,Yang, Fengjuan.,Li, Xiaogang.,Song, Yunpeng.,...&Hu, Xiangyang.(2010).Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity.JOURNAL OF PLANT PHYSIOLOGY,167(15),1298-1306.
MLA Wang, Lina,et al."Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity".JOURNAL OF PLANT PHYSIOLOGY 167.15(2010):1298-1306.

入库方式： OAI收割

来源：昆明植物研究所

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