Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)
文献类型：期刊论文
| 作者 | Li, Wei1,2,3; Xiang, Fen1; Zhong, Micai2; Zhou, Lingyun1; Liu, Hongyan1; Li, Saijun1; Wang, Xuewen2,4 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-05-10 |
| 卷号 | 7页码:1693 |
| DOI | 10.1038/s41598-017-01949-0 |
| 文献子类 | Article |
| 英文摘要 | Applied nitrogen (N) fertilizer significantly increases the leaf yield. However, most N is not utilized by the plant, negatively impacting the environment. To date, little is known regarding N utilization genes and mechanisms in the leaf production. To understand this, we investigated transcriptomes using RNA-seq and amino acid levels with N treatment in tea (Camellia sinensis), the most popular beverage crop. We identified 196 and 29 common differentially expressed genes in roots and leaves, respectively, in response to ammonium in two tea varieties. Among those genes, AMT, NRT and AQP for N uptake and GOGAT and GS for N assimilation were the key genes, validated by RT-qPCR, which expressed in a network manner with tissue specificity. Importantly, only AQP and three novel DEGs associated with stress, manganese binding, and gibberellin-regulated transcription factor were common in N responses across all tissues and varieties. A hypothesized gene regulatory network for N was proposed. A strong statistical correlation between key genes' expression and amino acid content was revealed. The key genes and regulatory network improve our understanding of the molecular mechanism of N usage and offer gene targets for plant improvement. |
| WOS关键词 | ROOT-ZONE PH ; ARABIDOPSIS-THALIANA ; AMMONIUM TRANSPORT ; AQUAPORIN HOMOLOGS ; NITRATE ; EXPRESSION ; NH4+ ; RICE ; RNA ; BIOSYNTHESIS |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000400886100033 |
| 资助机构 | Natural Science Foundation of Hunan Province, China(2016JJ2074) ; Innovation Team of the Hunan Academy of Agricultural Science, Chinese Academy of Sciences (CAS) ; Kunming Institute of Botany |
| 源URL | [http://ir.kib.ac.cn/handle/151853/51244]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Hunan Acad Agr Sci, Tea Res Inst, Natl Ctr Tea Improvement, Hunan Branch, 702 Yuanda Rd, Changsha 410125, Hunan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, 132 Lanhei Rd, Kunming 650201, Peoples R China 3.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Hunan, Peoples R China 4.Dept Genet, 120 E Green St, Athens, GA 30602 USA |
| 推荐引用方式 GB/T 7714 | Li, Wei,Xiang, Fen,Zhong, Micai,et al. Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)[J]. SCIENTIFIC REPORTS,2017,7:1693. |
| APA | Li, Wei.,Xiang, Fen.,Zhong, Micai.,Zhou, Lingyun.,Liu, Hongyan.,...&Wang, Xuewen.(2017).Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis).SCIENTIFIC REPORTS,7,1693. |
| MLA | Li, Wei,et al."Transcriptome and metabolite analysis identifies nitrogen utilization genes in tea plant (Camellia sinensis)".SCIENTIFIC REPORTS 7(2017):1693. |
入库方式： OAI收割
来源：昆明植物研究所
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