Seven new anthranilamide derivatives, acoapetalines A-G (1-7), including one new naturally occurring compound (7), together with six known ones (8-13) were isolated from the whole plants of Aconitum apetalum. Their structures were established on the basis of extensive spectroscopic analyses (IR, UV, HRESIMS, 1D and 2D NMR). All compounds were evaluated for their anti-tobacco mosaic virus (anti-TMV) activities. The results showed that compound 10 exhibited significant anti-TMV activity with an inhibition rate of 67.57% (100 mu g/mL), which was higher than that of ningnamycin (52.89%).