As an excellent catalyst toward hydrogen peroxide reduction, Fe-based nanomaterial like Fe-N-C configuration is promising candidate to be used for H2O2 sensing. In this work, a novel N-doped mesoporous carbon by atomically dispersed Fe-N dopant composite modified electrode (Fe/NOMC) was prepared by hard template approach and then fabricated non-enzymatic sensor was used for H2O2 determination. The morphology and property of Fe/NOMC composite were characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), small angle X-ray diffraction (XRD) spectrum and nitrogensorption measurement, respectively. The electrochemical sensing performance of the Fe/NOMC composite modified glassy carbon electrode (GCE) was studied by cyclic voltammetry and chronoamperometry methods. An excellent performance toward hydrogen peroxide reduction was achieved in a wide range of 8-23000 mu M, with limitation of detection (LOD) about 5 mu M and a correlation coefficient of 0.998, as well as a short response time (2 s) and good selectivity. (C) 2018 The Electrochemical Society.