Ultrasensitive Detection of Aminopeptidase N Activity in Urine and Cells with a Ratiometric Fluorescence Probe
文献类型：期刊论文
| 作者 | He, Xinyuan1,2; Xu, Yanhui1; Shi, Wen1,2; Ma, Huimin1,2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-03-07 |
| 卷号 | 89期号:5页码:3217-3221 |
| 英文摘要 | An ultrasensitive ratiometric fluorescent probe (CVN) has been designed and synthesized by incorporating alanine into the cresyl violet fluorophore. The probe shows ratiometric fluorescence response toward aminopeptidase N (APN) through the increase of fluorescent intensity ratio of 626/575 nm. The sensitivity of the probe is ultrahigh with a detection limit of 33 mu g/mL, which can quantify the contents of APN in 500-fold diluted human urine samples. Furthermore, by using ratiometric fluorescence imaging, the probe reveals significantly higher contents of APN in HepG2 cells than those in LO2 cells, which has been further used to distinguish these two types of cells in mixed cocultures. The probe could be of great importance for the APN-related disease diagnosis and pathophysiology elucidation. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/38281]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Xinyuan,Xu, Yanhui,Shi, Wen,et al. Ultrasensitive Detection of Aminopeptidase N Activity in Urine and Cells with a Ratiometric Fluorescence Probe[J]. ANALYTICAL CHEMISTRY,2017,89(5):3217-3221. |
| APA | He, Xinyuan,Xu, Yanhui,Shi, Wen,&Ma, Huimin.(2017).Ultrasensitive Detection of Aminopeptidase N Activity in Urine and Cells with a Ratiometric Fluorescence Probe.ANALYTICAL CHEMISTRY,89(5),3217-3221. |
| MLA | He, Xinyuan,et al."Ultrasensitive Detection of Aminopeptidase N Activity in Urine and Cells with a Ratiometric Fluorescence Probe".ANALYTICAL CHEMISTRY 89.5(2017):3217-3221. |
入库方式： OAI收割
来源：化学研究所
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