Capturing the photo-signaling state of a photoreceptor in a steady-state fashion by binding a transition metal complex
文献类型：期刊论文
| 作者 | Yu, Pengyun1,2; Song, Lei3; Qin, Jun3; Wang, Jianping1,2 |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2017-11-01 |
| 卷号 | 26期号:11页码:2249-2256 |
| 关键词 | Protein Intermediate Conformational Change Carbonyl Stretching Corms |
| 英文摘要 | Binding a small molecule to proteins causes conformational changes, but often to a limited extent. Here, we demonstrate that the interaction of a CO-releasing molecule (CORM3) with a photoreceptor photoactive yellow protein (PYP) drives large structural changes in the latter. The interaction of CORM3 and a mutant of PYP, Met100Ala, not only trigger the isomerization of its chromophore, p-coumaric acid, from its anionic trans configuration to a protonated cis configuration, but also increases the content of beta-sheet at the cost of alpha-helix and random coil in the secondary structure of the protein. The CORM3 derived Met100Ala is found to highly resemble the signaling state, which is one of the key photo-intermediates of this photoactive protein, in both protein local conformation and chromophore configuration. The organometallic reagents hold promise as protein engineering tools. This work highlights a novel approach to structurally accessing short lived intermediates of proteins in a steady-state fashion. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/39180]  |
| 专题 | 化学研究所_分子反应动力学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Mol React Dynam Lab, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.PHOENIX Ctr, State Key Lab Prote, Beijing Proteome Res Ctr, Beijing Inst Radiat Med,Natl Ctr Prot Sci, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Pengyun,Song, Lei,Qin, Jun,et al. Capturing the photo-signaling state of a photoreceptor in a steady-state fashion by binding a transition metal complex[J]. PROTEIN SCIENCE,2017,26(11):2249-2256. |
| APA | Yu, Pengyun,Song, Lei,Qin, Jun,&Wang, Jianping.(2017).Capturing the photo-signaling state of a photoreceptor in a steady-state fashion by binding a transition metal complex.PROTEIN SCIENCE,26(11),2249-2256. |
| MLA | Yu, Pengyun,et al."Capturing the photo-signaling state of a photoreceptor in a steady-state fashion by binding a transition metal complex".PROTEIN SCIENCE 26.11(2017):2249-2256. |
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来源：化学研究所
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