Interaction between G-Quadruplex and Zinc Cationic Porphyrin: The Role of the Axial Water
文献类型：期刊论文
| 作者 | Yao, Xiangzi1,3; Song, Di1; Qin, Tingxiao1,3; Yang, Chunfan2; Yu, Ze2; Li, Xiaohong2; Liu, Kunhui2; Su, Hongmei1,2,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-09-08 |
| 卷号 | 7 |
| 英文摘要 | The interaction of ligands with G-quadruplexes has attracted considerable attention due to its importance in molecular recognition and anticancer drugs design. Here, we utilize triplet excited state as a sensitive reporter to study the binding interaction of zinc cationic porphyrin (ZnTMPyP4) with three G-quadruplexes, AG(3)(T(2)AG(3))(3), (G(4)T(4)G(4))(2), and (TG(4)T)4. By monitoring the triplet decay dynamics of ZnTMPyP4 with transient absorption spectroscopy, the coexisted binding modes via p-p stacking of porphyrin macrocycle and the G-quartets are allowed to be identified quantitatively, which involve intercalation (25% and 36%) versus end-stacking (75% and 64%) for AG(3)(T(2)AG(3))(3) and (G(4)T(4)G(4))(2), and end-stacking (23%) versus partial intercalation (77%) for (TG(4)T)4. It is shown that the steric hindrance of the axial water decreases greatly the percentage of intercalation. Further, a rapid assessment of binding stoichiometry is fulfilled by measuring the triplet decay dynamics under various [G-quadruplex]/[ZnTMPyP4] ratios. The binding stoichiometric ratios of G-quadruplex/ZnTMPyP4 are 1:2 for AG(3)(T(2)AG(3))(3), 1:1 for (G(4)T(4)G(4)) 2, and 1:2 for (TG(4)T)4, which agree well with results obtained by the conventional method of continuous variation analysis. These results reveal a clear scenario of G-quadruplex/ZnTMPyP4 interaction and provide mechanistic insights for the application of anticancer drug designs using G-quadruplex as target. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/39306]  |
| 专题 | 化学研究所_光化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China 2.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Xiangzi,Song, Di,Qin, Tingxiao,et al. Interaction between G-Quadruplex and Zinc Cationic Porphyrin: The Role of the Axial Water[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Yao, Xiangzi.,Song, Di.,Qin, Tingxiao.,Yang, Chunfan.,Yu, Ze.,...&Su, Hongmei.(2017).Interaction between G-Quadruplex and Zinc Cationic Porphyrin: The Role of the Axial Water.SCIENTIFIC REPORTS,7. |
| MLA | Yao, Xiangzi,et al."Interaction between G-Quadruplex and Zinc Cationic Porphyrin: The Role of the Axial Water".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。