Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor
文献类型：期刊论文
| 作者 | Jin, Yulong1,2; Xie, Yunfeng1,2,3; Wu, Kui1,2; Huang, Yanyan1,2; Wang, Fuyi1,2; Zhao, Rui1,2 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2017-03-15 |
| 卷号 | 9期号:10页码:8490-8497 |
| 关键词 | Dna Damage Piezoelectric Mass Biosensor High-mobility-group Box 1 Molecular Recognition Kinetic Analysis |
| 英文摘要 | The binding events between damaged DNA and recognition biomolecules are of great interest for understanding the activity of DNA-damaging drugs and the related DNA repair networks. Herein, a simple and sensitive sensor system was tailored for real-time probing of the dynamic molecular recognition between cisplatin-damaged-DNA (cisPt-DNA) and a cellular responsive protein, high mobility-group box 1 (HMGB1). By integration of flow injection analysis (FIA) with quartz crystal microbalance (QCM), the interaction time-course of cisPt-DNA and HMGB1 domain A (HMGB1a) was investigated. The highly specific sensing interface was carefully designed and fabricated using cisPt-DNA as recognition element. A hybrid self-assembled monolayer consisting of cysteamine and mercaptohexanol was introduced to resist nonspecific adsorption. The calculated kinetic parameters (k(ass) and k(diss)) and the dissociation constant (KD) demonstrated the rapid recognition and tight binding of HMGBla toward cisPt-DNA. Molecular docking was employed to simulate the complex formed by cisPt-DNA and HMGB1a. The tight binding of such a DNA-damage responsive complex is appealing for the downstream molecular recognition event related to the resistance to DNA repair. This continuous-flow QCM biosensor is an ideal tool for studying specific interactions between drug-damaged-DNAs and their recognition proteins in a physiological-relevant environment, and will provide a potential sensor platform for rapid screening and evaluating metal anticancer drugs. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/38257]  |
| 专题 | 化学研究所_分析测试中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, CAS Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.China Natl Cereals Oils & Foodstuffs Corp, Beijing 100020, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Yulong,Xie, Yunfeng,Wu, Kui,et al. Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(10):8490-8497. |
| APA | Jin, Yulong,Xie, Yunfeng,Wu, Kui,Huang, Yanyan,Wang, Fuyi,&Zhao, Rui.(2017).Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor.ACS APPLIED MATERIALS & INTERFACES,9(10),8490-8497. |
| MLA | Jin, Yulong,et al."Probing the Dynamic Interaction between Damaged DNA and a Cellular Responsive Protein Using a Piezoelectric Mass Biosensor".ACS APPLIED MATERIALS & INTERFACES 9.10(2017):8490-8497. |
入库方式： OAI收割
来源：化学研究所
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