Dual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH &ITin Vivo&IT
文献类型：期刊论文
| 作者 | Ma, Tiancong; Hou, Yi; Zeng, Jianfeng; Liu, Chunyan; Zhang, Peisen; Jing, Lihong; Shangguan, Dihua; Gao, Mingyuan |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | JAN 10 2018 |
| 卷号 | 140期号:1页码:211-218 |
| 英文摘要 | The abnormal expression of tumor-associated proteases and lowered extracellular pH are important signatures strongly associated with cancer invasion, progression, and metastasis. However, their malignant effects were mainly identified using cell and tissue studies. To noninvasively visualize the heterogeneous distribution of these abnormal indicators in vivo and further disclose their collective behaviors, a target-triggered fluorescent nanoprobe composed of a ratiometric pH-sensitive dye, a near-infrared dye (Cy5.5), and biocompatible Fe3O4 nanoparticles was constructed. The pH-sensitive dye was linked through a peptide substrate of matrix metalloprotease-9 (MMP-9) with Fe3O4 nanoparticles to establish a Forster resonance energy transfer (FRET) system for sensing the pH of the tumor microenvironment. Cy5.5 served as an internal reference for forming a secondary ratiometric fluorescent system together with the activated pH dye to enable the visualization of protease activities in vivo. Extensive imaging studies using a mouse model of human colon cancer revealed that the overexpression of MMP-9 and abnormal microenvironmental pH quantitatively visualized by this dual-ratiometric probe are spatially heterogeneous and synergistically guide the tumor invasion in vivo. |
| WOS记录号 | WOS:000422813300047 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/38855]  |
| 专题 | 化学研究所_其它 |
| 推荐引用方式 GB/T 7714 | Ma, Tiancong,Hou, Yi,Zeng, Jianfeng,et al. Dual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH &ITin Vivo&IT[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,JAN ,140(1):211-218. |
| APA | Ma, Tiancong.,Hou, Yi.,Zeng, Jianfeng.,Liu, Chunyan.,Zhang, Peisen.,...&Gao, Mingyuan.(JAN ).Dual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH &ITin Vivo&IT.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,140(1),211-218. |
| MLA | Ma, Tiancong,et al."Dual-Ratiometric Target-Triggered Fluorescent Probe for Simultaneous Quantitative Visualization of Tumor Microenvironment Protease Activity and pH &ITin Vivo&IT".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 140.1(JAN ):211-218. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。