Detection of G-Quadruplex Structures Formed by G-Rich Sequences from Rice Genome and Transcriptome Using Combined Probes
文献类型：期刊论文
| 作者 | Chang, Tianjun1; Li, Weiguo1; Ding, Zhan1; Cheng, Shaofei1; Liang, Kun1; Liu, Xiangjun2,3; Bin, Tao2,3; Shangguan, Dihua2,3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-08-01 |
| 卷号 | 89期号:15页码:8162-8169 |
| 英文摘要 | Putative G-quadruplex (G4) forming sequences (PQS) are highly prevalent in the genome and transcriptome of various organisms and are considered as potential regulation elements in many biological processes by forming G4 structures. The formation of G4 structures highly depends on the sequences and the environment. In most cases, it is difficult to predict G4 formation by PQS, especially PQS containing G2 tracts. Therefore, the experimental identification of G4 formation is essential in the study of G4-related biological functions. Herein, we report a rapid and simple method for the detection of G4 structures by using a pair of complementary reporters, hemin and BMSP. This method was applied to detect G4 structures formed by PQS (DNA and RNA) searched in the genome and transcriptome of Oryza sativa. Unlike most of the reported G4 probes that only recognize part of G4 structures, the proposed method based on combined probes positively responded to almost all G4 conformations, including parallel, antiparallel, and mixed/hybrid G4, but did not respond to non-G4 sequences. This method shows potential for high-throughput identification of G4 structures in genome and transcriptome. Furthermore, BMSP was observed to drive some PQS to form more stable G4 structures or induce the G4 formation of some PQS that cannot form G4 in normal physiological conditions, which may provide a powerful molecular tool for gene regulation. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/39207]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Henan Polytech Univ, Inst Resources & Environm, Dept Biol, Jiaozuo 454000, Peoples R China 2.Chinese Acad Sci, Inst Chem,Beijing Natl Lab Mol Sci, CAS Res Educ Ctr Excellence Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Tianjun,Li, Weiguo,Ding, Zhan,et al. Detection of G-Quadruplex Structures Formed by G-Rich Sequences from Rice Genome and Transcriptome Using Combined Probes[J]. ANALYTICAL CHEMISTRY,2017,89(15):8162-8169. |
| APA | Chang, Tianjun.,Li, Weiguo.,Ding, Zhan.,Cheng, Shaofei.,Liang, Kun.,...&Shangguan, Dihua.(2017).Detection of G-Quadruplex Structures Formed by G-Rich Sequences from Rice Genome and Transcriptome Using Combined Probes.ANALYTICAL CHEMISTRY,89(15),8162-8169. |
| MLA | Chang, Tianjun,et al."Detection of G-Quadruplex Structures Formed by G-Rich Sequences from Rice Genome and Transcriptome Using Combined Probes".ANALYTICAL CHEMISTRY 89.15(2017):8162-8169. |
入库方式： OAI收割
来源：化学研究所
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