Hyaluronic acid modification of RNase A and its intracellular delivery using lipid-like nanoparticles
文献类型：期刊论文
| 作者 | Wang, Xiaoying1,2; Li, Yamin1; Li, Quanshun1,3; Neufeld, Caleb I.1; Pouli, Dimitra1; Sun, Shuo1; Yang, Liu1; Deng, Pu1; Wang, Ming1,4; Georgakoudi, Irene1 |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2017-10-10 |
| 卷号 | 263页码:39-45 |
| 关键词 | Targeted Protein Delivery Lipidoids Protein Engineering Cd44 Hyaluronic Acid |
| 英文摘要 | Developing safe and effective nanosystems to deliver active and therapeutic proteins to targeted cells and organs is an important tool for many biomedical applications. We present here a simple and efficient strategy for this purpose: delivering hyaluronic acid (HA)-modified RNase A (RNase A-HA) in nanocomplex with cationic lipid-like molecules (lipidoids) to cancer cells, resulting in targeted inhibition of cancer proliferation. The chemical conjugation of RNase A with HA both increased the supramolecular interaction with carrier lipidoids, promoting protein encapsulation efficacy, and facilitated cancer cell targeting via interaction with overexpressed CD44. Through confocal laser scanning microscopy and flow cytometry analysis, we demonstrated that protein/lipidoid nanoparticles could facilely enter cells with high CD44 expression, and inhibit cell proliferation in a dose-dependent manner. (C) 2017 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/41054]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA 2.Jinan Univ, Dept Biomed Engn, Guangzhou 510632, Guangdong, Peoples R China 3.Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Jilin, Peoples R China 4.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaoying,Li, Yamin,Li, Quanshun,et al. Hyaluronic acid modification of RNase A and its intracellular delivery using lipid-like nanoparticles[J]. JOURNAL OF CONTROLLED RELEASE,2017,263:39-45. |
| APA | Wang, Xiaoying.,Li, Yamin.,Li, Quanshun.,Neufeld, Caleb I..,Pouli, Dimitra.,...&Xu, Qiaobing.(2017).Hyaluronic acid modification of RNase A and its intracellular delivery using lipid-like nanoparticles.JOURNAL OF CONTROLLED RELEASE,263,39-45. |
| MLA | Wang, Xiaoying,et al."Hyaluronic acid modification of RNase A and its intracellular delivery using lipid-like nanoparticles".JOURNAL OF CONTROLLED RELEASE 263(2017):39-45. |
入库方式： OAI收割
来源：化学研究所
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