Surface Plasmon Resonance Imaging Detection of Sub-femtomolar MicroRNA
文献类型：期刊论文
| 作者 | Hu, Feichi1,2; Xu, Jiying1; Chen, Yi1,2,3 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-09-19 |
| 卷号 | 89期号:18页码:10071-10077 |
| 英文摘要 | MicroRNA (miRNA) is a promising new type of biomarkers but at a low fM level and hard to be analyzed. Herein proposed is an innovated surface plasmon resonance imaging (SPRi) method merged with a novel in plane and vertical signal amplification strategy, that is, orthogonal signal amplification to enable a direct determination of sub-fM miRNA-15a (a multiple tumor diagnostic biomarker). The core idea is to add more Mass on a target sample spot first along the surficial direction, then upward from the surface. In detection of miRNA, this was realized by coupling. a miRNA-initiated surficial cyclic DNA-DNA hybridization reaction with a DNA initiated upward cyclic polymerization reaction. A perfect SPRi sensing chip with isolated gold islands bordered by hydrophobic CYTOP was fabricated and used to obtain high-quality chip with low fabrication difficulty: As a result, SPRi contrast largely increases, able to reach a limit of detection and limit of quantification down to 0.56 and 5fM for miRNA-15a, about 10(7)-fold improvement of sensitivity compared with a common SPRi detection. The method could quantify standard miRNA-15a spiked in human serum with an ideal recovery ranging from 98.6% to 104.9% and was validated to be applicable to the direct determination of miRNA-15a in healthy and cancer huinan serums. The orderly and controllable in situ sensitizing strategy is powerful and readily extendable to detection of other miRNAs. |
| 语种 | 英语 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/41109]  |
| 专题 | 化学研究所_活体分析化学实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, CAS Res Educ Ctr Excellence Mol Sci, Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Feichi,Xu, Jiying,Chen, Yi. Surface Plasmon Resonance Imaging Detection of Sub-femtomolar MicroRNA[J]. ANALYTICAL CHEMISTRY,2017,89(18):10071-10077. |
| APA | Hu, Feichi,Xu, Jiying,&Chen, Yi.(2017).Surface Plasmon Resonance Imaging Detection of Sub-femtomolar MicroRNA.ANALYTICAL CHEMISTRY,89(18),10071-10077. |
| MLA | Hu, Feichi,et al."Surface Plasmon Resonance Imaging Detection of Sub-femtomolar MicroRNA".ANALYTICAL CHEMISTRY 89.18(2017):10071-10077. |
入库方式： OAI收割
来源：化学研究所
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