An Exonuclease III-Powered, On-Particle Stochastic DNA Walker
文献类型：期刊论文
| 作者 | Qu, XM; Zhu, D; Yao, GB; Su, S; Chao, J; Liu, HJ; Zuo, XL; Wang, LH; Shi, JY; Wang, LH |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2017 |
| 卷号 | 56期号:7页码:1855-1858 |
| 关键词 | Brownian Motions Dna Nanotechnology Enzymes Molecular Machines Nanoparticles |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201611777 |
| 文献子类 | 期刊论文 |
| 英文摘要 | DNA-based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonucleaseIII (ExoIII)-powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)-based 3D track. The motion is propelled by unidirectional ExoIII digestion of hybridized DNA tracks in a burnt-bridge mechanism. The operation of this ExoIII-propelled DNA walker was monitored in real time and at the single-particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA-based stochastic DNA walker by exploiting movement-triggered cascade signal amplification. |
| 语种 | 英语 |
| WOS记录号 | WOS:000395554900026 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/27252]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Qu, XM,Zhu, D,Yao, GB,et al. An Exonuclease III-Powered, On-Particle Stochastic DNA Walker[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(7):1855-1858. |
| APA | Qu, XM.,Zhu, D.,Yao, GB.,Su, S.,Chao, J.,...&Fan, CH.(2017).An Exonuclease III-Powered, On-Particle Stochastic DNA Walker.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(7),1855-1858. |
| MLA | Qu, XM,et al."An Exonuclease III-Powered, On-Particle Stochastic DNA Walker".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.7(2017):1855-1858. |
入库方式： OAI收割
来源：上海应用物理研究所
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