Expression and radiolabeling of Cas9 protein
文献类型：期刊论文
| 作者 | Yan, QL; Kong, HT; Xia, K; Zhang, Y; Aladlbahi, A; Shi, JY; Wang, LH; Fan, CH; Zhao, Y; Zhu, Y |
| 刊名 | NUCLEAR SCIENCE AND TECHNIQUES
 |
| 出版日期 | 2017 |
| 卷号 | 28期号:1页码:- |
| 关键词 | Cas9 Radiolabeling I-125 Labeling Yield Stability |
| ISSN号 | 1001-8042 |
| DOI | 10.1007/s41365-016-0165-8 |
| 文献子类 | 期刊论文 |
| 英文摘要 | As a robust platform for genome editing, CRISPR/Cas9 is currently being explored for engineering biology or therapeutics, yet means for quantitative detection of Cas9 proteins remain to be fully realized. Here, we expressed Cas9 proteins and developed a novel detection method that traced Cas9 based on radiolabeled iodine. Through optimizing the reaction conditions of reaction time, temperature and cycles, we obtained I-125-Cas9 of high labeling yield. The prepared I-125-Cas9 was stable in various media and preserved excellent genome editing efficiency. Thus, our strategy provides a convenient and efficient tool for further tracing biological behaviors of Cas9 proteins in living systems. |
| 语种 | 英语 |
| WOS记录号 | WOS:000393150100007 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/27319]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Yan, QL,Kong, HT,Xia, K,et al. Expression and radiolabeling of Cas9 protein[J]. NUCLEAR SCIENCE AND TECHNIQUES,2017,28(1):-. |
| APA | Yan, QL.,Kong, HT.,Xia, K.,Zhang, Y.,Aladlbahi, A.,...&Zhu, Y.(2017).Expression and radiolabeling of Cas9 protein.NUCLEAR SCIENCE AND TECHNIQUES,28(1),-. |
| MLA | Yan, QL,et al."Expression and radiolabeling of Cas9 protein".NUCLEAR SCIENCE AND TECHNIQUES 28.1(2017):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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