DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis
文献类型：期刊论文
| 作者 | Song, P; Ye, DK; Zuo, XL; Li, J; Wang, JB; Liu, HJ; Hwang, MT; Chao, J; Su, S; Wang, LH |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2017 |
| 卷号 | 17期号:9页码:5193-5198 |
| 关键词 | Hybridization Chain-reaction Cancer-cells Capture Release Nanostructures Selection Platform Fate Chip |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/acs.nanolett.7b01006 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Circulating tumor cells (CTCs) contain molecular information on the primary tumor and can be used for predictive cancer diagnostics. Capturing rare live CTCs and their quantification in whole blood remain technically challenging. Here we report an aptamer-trigger clamped hybridization chain reaction (atcHCR) method for in situ identification and subsequent cloaking/decloaking of CTCs by porous DNA hydrogels. These decloaked CTCs were then used for live cell analysis. In our design, a DNA staple strand with aptamer-toehold biblocks specifically recognizes epithelial cell adhesion molecule (EpCAM) on the CTC surface that triggers subsequent atcHCR via toehold-initiated branch migration. Porous DNA hydrogel based-cloaking of single/cluster of CTCs allows capturing of living CTCs directly with minimal cell damage. The ability to identify a low number of CTCs in whole blood by DNA hydrogel cloaking would allow high sensitivity and specificity for diagnosis in clinically relevant settings. More significantly, decloaking of CTCs using controlled and defined chemical stimuli can release living CTCs without damages for subsequent culture and live cell analysis. We expect this liquid biopsy tool to open new powerful and effective routes for cancer diagnostics and therapeutics. |
| WOS关键词 | HYBRIDIZATION CHAIN-REACTION ; CANCER-CELLS ; CAPTURE ; RELEASE ; NANOSTRUCTURES ; SELECTION ; PLATFORM ; FATE ; CHIP |
| 语种 | 英语 |
| WOS记录号 | WOS:000411043500003 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28614]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Song, P,Ye, DK,Zuo, XL,et al. DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis[J]. NANO LETTERS,2017,17(9):5193-5198. |
| APA | Song, P.,Ye, DK.,Zuo, XL.,Li, J.,Wang, JB.,...&Fan, CH.(2017).DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis.NANO LETTERS,17(9),5193-5198. |
| MLA | Song, P,et al."DNA Hydrogel with Aptamer-Toehold-Based Recognition, Cloaking, and Decloaking of Circulating Tumor Cells for Live Cell Analysis".NANO LETTERS 17.9(2017):5193-5198. |
入库方式： OAI收割
来源：上海应用物理研究所
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