DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection
文献类型：期刊论文
| 作者 | Qi, L; Xiao, MS; Wang, XW; Wang, C; Wang, LH; Song, SP; Qu, XM; Li, L; Shi, JY; Pei, H |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 89期号:18页码:9850-9856 |
| 关键词 | Signal Amplification Sensitive Detection Ultrasensitive Detection Living Cells Small Rnas Sers Cancer Scattering Platform Paper |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.7b01861 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies. The resulting AgNPs were further encoded with DNA probes to serve as effective surface-enhanced Raman scattering (SERS) probes. The analytical device was then fabricated on a single piece of SERS probes loaded paper-based substrate and assembled based on the principles of origami. The addition of the target analyte amplifies the Raman signals on DNA-encoded AgNPs through a target-dependent, sequence specific DNA hybridization assembly. This simple and low-cost analytical device is generic and applicable to a variety of miRNAs, allowing detection sensitivity down to 1 pM and assay time within 15 min, and therefore holds promising applications in point-of-care diagnostics. |
| WOS关键词 | SIGNAL AMPLIFICATION ; SENSITIVE DETECTION ; ULTRASENSITIVE DETECTION ; LIVING CELLS ; SMALL RNAS ; SERS ; CANCER ; SCATTERING ; PLATFORM ; PAPER |
| 语种 | 英语 |
| WOS记录号 | WOS:000411549100035 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28616]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Qi, L,Xiao, MS,Wang, XW,et al. DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection[J]. ANALYTICAL CHEMISTRY,2017,89(18):9850-9856. |
| APA | Qi, L.,Xiao, MS.,Wang, XW.,Wang, C.,Wang, LH.,...&Pei, H.(2017).DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection.ANALYTICAL CHEMISTRY,89(18),9850-9856. |
| MLA | Qi, L,et al."DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection".ANALYTICAL CHEMISTRY 89.18(2017):9850-9856. |
入库方式： OAI收割
来源：上海应用物理研究所
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