Docking of Antibodies into the Cavities of DNA Origami Structures
文献类型：期刊论文
| 作者 | Ouyang, XY; De Stefano, M; Krissanaprasit, A; Kodal, ALB; Rosen, CB; Liu, TQ; Helmig, S; Fan, CH; Gothelf, KV |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2017 |
| 卷号 | 56期号:46页码:14423-14427 |
| 关键词 | Self-assembled Nanostructures Surface-plasmon Resonance Histidine-tagged Proteins Oriented Immobilization Igg Immobilization 3d Dna Purification Binding Shapes Arrays |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.201706765 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Immobilized antibodies are extensively employed for medical diagnostics, such as in enzyme-linked immuno-sorbent assays. Despite their widespread use, the ability to control the orientation of immobilized antibodies on surfaces is very limited. Herein, we report a method for the covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNAorigami structures. Two tris(NTA)modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysine residues. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) confirmed the efficient immobilization of the antibodies in the origami structures. This increased control over the orientation of antibodies in nanostructures and on surfaces has the potential to direct the interactions between antibodies and targets and to provide more regular surface assemblies of antibodies. |
| WOS关键词 | SELF-ASSEMBLED NANOSTRUCTURES ; SURFACE-PLASMON RESONANCE ; HISTIDINE-TAGGED PROTEINS ; ORIENTED IMMOBILIZATION ; IGG IMMOBILIZATION ; 3D DNA ; PURIFICATION ; BINDING ; SHAPES ; ARRAYS |
| 语种 | 英语 |
| WOS记录号 | WOS:000414764600011 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28618]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Ouyang, XY,De Stefano, M,Krissanaprasit, A,et al. Docking of Antibodies into the Cavities of DNA Origami Structures[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(46):14423-14427. |
| APA | Ouyang, XY.,De Stefano, M.,Krissanaprasit, A.,Kodal, ALB.,Rosen, CB.,...&Gothelf, KV.(2017).Docking of Antibodies into the Cavities of DNA Origami Structures.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(46),14423-14427. |
| MLA | Ouyang, XY,et al."Docking of Antibodies into the Cavities of DNA Origami Structures".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.46(2017):14423-14427. |
入库方式： OAI收割
来源：上海应用物理研究所
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