PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe
文献类型：期刊论文
| 作者 | Yang, X; Wen, YL; Wang, LL; Zhou, CQ; Li, Q; Xu, L; Li, LY; Shi, JY; Lal, R; Ren, SZ |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:44页码:38281-38287 |
| 关键词 | Sequence-specific Detection Highly Sensitive Detection Ovarian-cancer Incidence Gold Nanoparticles Electrochemical Detection Signal Amplification Biosensing Interface Protein-detection Magnetic Beads Chain-reaction |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.7b11994 |
| 文献子类 | 期刊论文 |
| 英文摘要 | A "sandwich-like" biosensor was developed on the basis of the magnetic bead platform for sensitive detection of breast cancer 1 (BRCA1) DNA. In the present study, a tetrahedron-structured reporter probe (TSRP) was designed, in which 3 vertices of the tetrahedron were labeled with digoxin (Dig), and the other one was labeled with a detection probe. TSRP here provided accurate enzyme loading and well-organized spatial arrangement for optimized signal amplification. The detection limit of this biosensor was as low as 10 fM, which is at least 4 orders of magnitude lower than that of the single DNA probe (100 pM), and the signal gain was 2 times higher than the analysis using three one-dimensional (1D) reporter probes. We could distinguish DNA sequences with only 1 base mismatch, and the performance of our TSRP biosensor was proven to be equally good in both PCR products and real fetal calf serum (FCS) sample as in buffer. We believe this work provided a novel avenue for the development of signal amplification strategies. |
| WOS关键词 | SEQUENCE-SPECIFIC DETECTION ; HIGHLY SENSITIVE DETECTION ; OVARIAN-CANCER INCIDENCE ; GOLD NANOPARTICLES ; ELECTROCHEMICAL DETECTION ; SIGNAL AMPLIFICATION ; BIOSENSING INTERFACE ; PROTEIN-DETECTION ; MAGNETIC BEADS ; CHAIN-REACTION |
| 语种 | 英语 |
| WOS记录号 | WOS:000415140800015 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28767]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Yang, X,Wen, YL,Wang, LL,et al. PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(44):38281-38287. |
| APA | Yang, X.,Wen, YL.,Wang, LL.,Zhou, CQ.,Li, Q.,...&Liu, G.(2017).PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe.ACS APPLIED MATERIALS & INTERFACES,9(44),38281-38287. |
| MLA | Yang, X,et al."PCR-Free Colorimetric DNA Hybridization Detection Using a 3D DNA Nanostructured Reporter Probe".ACS APPLIED MATERIALS & INTERFACES 9.44(2017):38281-38287. |
入库方式： OAI收割
来源：上海应用物理研究所
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