Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System
文献类型：期刊论文
| 作者 | Qu, XM; Li, M; Zhang, HB; Lin, CL; Wang, F; Xiao, MS; Zhou, Y; Shi, J; Aldalbahi, A; Pei, H |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:37页码:31568-31575 |
| 关键词 | Tetrahedral Dna Nanostructures Microarray Analysis Cancer-cells Signal Amplification Gold Nanoparticles Nucleic-acid Chip Microrna Insights Platform |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.7b10116 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ the thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs. The combination of thermal denaturation and laser-induced fluorescence detection technique enables real-time continuous identification of multiple samples (<10 min per sample). As a proof of concept, we have demonstrated that two DNA targets of fungal pathogens (Botrytis cinerea and Didymella bryoniae) can be sequentially analyzed using our rapid microfluidic bioassay system, which provides a new paradigm in the design of microfluidic bioassay system and will be valuable for chemical and biomedical analysis. |
| WOS关键词 | TETRAHEDRAL DNA NANOSTRUCTURES ; MICROARRAY ANALYSIS ; CANCER-CELLS ; SIGNAL AMPLIFICATION ; GOLD NANOPARTICLES ; NUCLEIC-ACID ; CHIP ; MICRORNA ; INSIGHTS ; PLATFORM |
| 语种 | 英语 |
| WOS记录号 | WOS:000411771400038 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28794]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Qu, XM,Li, M,Zhang, HB,et al. Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(37):31568-31575. |
| APA | Qu, XM.,Li, M.,Zhang, HB.,Lin, CL.,Wang, F.,...&Li, L.(2017).Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System.ACS APPLIED MATERIALS & INTERFACES,9(37),31568-31575. |
| MLA | Qu, XM,et al."Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System".ACS APPLIED MATERIALS & INTERFACES 9.37(2017):31568-31575. |
入库方式： OAI收割
来源：上海应用物理研究所
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