Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging
文献类型：期刊论文
| 作者 | Liu, MM; Li, Q; Liang, L; Li, J; Wang, K; Li, JJ; Lv, M; Chen, N; Song, HY; Lee, J |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:-页码:- |
| 关键词 | Clathrin-mediated Endocytosis Cell Mechanisms Microscopy Endosomes Dynamics Platform Motors Cancer Virus |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/ncomms15646 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management. |
| WOS关键词 | CLATHRIN-MEDIATED ENDOCYTOSIS ; CELL ; MECHANISMS ; MICROSCOPY ; ENDOSOMES ; DYNAMICS ; PLATFORM ; MOTORS ; CANCER ; VIRUS |
| 语种 | 英语 |
| WOS记录号 | WOS:000402395100001 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28796]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Liu, MM,Li, Q,Liang, L,et al. Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging[J]. NATURE COMMUNICATIONS,2017,8(-):-. |
| APA | Liu, MM.,Li, Q.,Liang, L.,Li, J.,Wang, K.,...&Fan, CH.(2017).Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging.NATURE COMMUNICATIONS,8(-),-. |
| MLA | Liu, MM,et al."Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging".NATURE COMMUNICATIONS 8.-(2017):-. |
入库方式： OAI收割
来源：上海应用物理研究所
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