Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization
文献类型：期刊论文
| 作者 | Gao, ZS; Deng, SH; Li, J; Wang, K; Li, JJ; Wang, LH; Fan, CH |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 60期号:10页码:1305-1309 |
| 关键词 | Living Brain-slices Sted Microscopy Fluorescence Microscopy Stimulated-emission Pericentriolar Material Electron-microscopy Nanoscale Light Scattering Reveals |
| ISSN号 | 1674-7291 |
| DOI | 10.1007/s11426-016-9028-5 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Stimulated emission depletion (STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization, our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging. |
| WOS关键词 | LIVING BRAIN-SLICES ; STED MICROSCOPY ; FLUORESCENCE MICROSCOPY ; STIMULATED-EMISSION ; PERICENTRIOLAR MATERIAL ; ELECTRON-MICROSCOPY ; NANOSCALE ; LIGHT ; SCATTERING ; REVEALS |
| 语种 | 英语 |
| WOS记录号 | WOS:000415714400006 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/28836]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Gao, ZS,Deng, SH,Li, J,et al. Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization[J]. SCIENCE CHINA-CHEMISTRY,2017,60(10):1305-1309. |
| APA | Gao, ZS.,Deng, SH.,Li, J.,Wang, K.,Li, JJ.,...&Fan, CH.(2017).Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization.SCIENCE CHINA-CHEMISTRY,60(10),1305-1309. |
| MLA | Gao, ZS,et al."Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization".SCIENCE CHINA-CHEMISTRY 60.10(2017):1305-1309. |
入库方式： OAI收割
来源：上海应用物理研究所
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