In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time
文献类型：期刊论文
| 作者 | Wang, ZJ; Luo, Y; Xie, XD; Hu, XJ; Song, HY; Zhao, Y; Shi, JY; Wang, LH; Glinsky, G; Chen, N |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2018 |
| 卷号 | 57期号:4页码:972-976 |
| ISSN号 | 1433-7851 |
| 关键词 | Green Fluorescent Protein Messenger-rna Living Cells Spinach Dynamics Reveals Probes Translation Mimics |
| DOI | 10.1002/anie.201707795 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Direct cellular imaging of the localization and dynamics of biomolecules helps to understand their function and reveals novel mechanisms at the single-cell resolution. In contrast to routine fluorescent-protein-based protein imaging, technology for RNA imaging remains less well explored because of the lack of enabling technology. Herein, we report the development of an aptamer-initiated fluorescence complementation (AiFC) method for RNA imaging by engineering a green fluorescence protein (GFP)-mimicking turn-on RNA aptamer, Broccoli, into two split fragments that could tandemly bind to target mRNA. When genetically encoded in cells, endogenous mRNA molecules recruited Split-Broccoli and brought the two fragments into spatial proximity, which formed a fluorophore-binding site in situ and turned on fluorescence. Significantly, we demonstrated the use of AiFC for high-contrast and real-time imaging of endogenous RNA molecules in living mammalian cells. We envision wide application and practical utility of this enabling technology to in vivo single-cell visualization and mechanistic analysis of macromolecular interactions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000428208700017 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/29045]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Wang, ZJ 2.Luo, Y 3.Xie, XD 4.Hu, XJ 5.Song, HY 6.Zhao, Y 7.Shi, JY 8.Wang, LH 9.Glinsky, G 10.Chen, N |
| 推荐引用方式 GB/T 7714 | Wang, ZJ,Luo, Y,Xie, XD,et al. In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018,57(4):972-976. |
| APA | Wang, ZJ.,Luo, Y.,Xie, XD.,Hu, XJ.,Song, HY.,...&Fan, CH.(2018).In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,57(4),972-976. |
| MLA | Wang, ZJ,et al."In Situ Spatial Complementation of Aptamer-Mediated Recognition Enables Live-Cell Imaging of Native RNA Transcripts in Real Time".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.4(2018):972-976. |
入库方式： OAI收割
来源：上海应用物理研究所
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