MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification
文献类型：期刊论文
| 作者 | Xiao, MS; Man, TT; Zhu, CF; Pei, H; Shi, JY; Li, L; Qu, XM; Shen, XZ; Li, J |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2018 |
| 卷号 | 10期号:9页码:7852-7858 |
| 关键词 | Metal Dichalcogenide Nanosheets Dna Nanostructure Molecular Beacons Graphene Oxide Isothermal Amplification Ultrasensitive Detection Ws2 Nanosheet Cancer-cells Fluorescence Identification |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.7b18984 |
| 文献子类 | 期刊论文 |
| 英文摘要 | MicroRNAs (miRNAs) play significant regulatory roles in physiologic and pathologic processes and are considered as important biomarkers for disease diagnostics and therapeutics. Simple, fast, sensitive, and selective detection of miRNAs, however, is challenged by their short length, low abundance, susceptibility to degradation, and homogenous sequence. Here, we report a novel design of nanoprobes for highly sensitive and selective detection of miRNAs based on MoSrloaded molecular beacons (MBs) and duplex-specific nuclease (DSN)-mediated signal amplification (DSNMSA). We show that MoS, nanosheets not only exhibit high affinity toward MBs but also act as an efficient quencher for absorbed MBs. The strong fluorescence-quenching ability of MoS2 in combination with cyclic DSNMSA contributes to the superior sensitivity of our method, with a limit of detection 4 orders of magnitude lower than that of traditional hybridization methods. Moreover, the nanoprobes also show high selectivity for discriminating homogenous miRNA sequences with one-base differences because of the discrimination ability of MBs and DSN. Furthermore, we demonstrate that the MoS2-loaded MB nanoprobes can be utilized for multiplexed detection of miRNAs. Given its high sensitivity and specificity, as well as the multiplexed function; this novel method as an effective tool shows a great promise for simultaneous quantitative analysis of multiple miRNAs in biomedical research and clinical diagnosis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000427204100035 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/29077]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Xiao, MS 2.Man, TT 3.Zhu, CF 4.Pei, H 5.Shi, JY 6.Li, L 7.Qu, XM 8.Shen, XZ 9.Li, J |
| 推荐引用方式 GB/T 7714 | Xiao, MS,Man, TT,Zhu, CF,et al. MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(9):7852-7858. |
| APA | Xiao, MS.,Man, TT.,Zhu, CF.,Pei, H.,Shi, JY.,...&Li, J.(2018).MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification.ACS APPLIED MATERIALS & INTERFACES,10(9),7852-7858. |
| MLA | Xiao, MS,et al."MoS2 Nanoprobe for MicroRNA Quantification Based on Duplex-Specific Nuclease Signal Amplification".ACS APPLIED MATERIALS & INTERFACES 10.9(2018):7852-7858. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。