NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA
文献类型：期刊论文
| 作者 | Deng, YJ; Feng, L; Zhou, H; Xiao, X; Wang, FP; Liu, XP |
| 刊名 | DNA REPAIR
 |
| 出版日期 | 2018 |
| 卷号 | 65页码:54-63 |
| 关键词 | Prime Transcription Initiation Exoribonuclease Rnase-r Escherichia-coli Crystal-structure Messenger-rna Mycobacterium-tuberculosis Bacillus-subtilis Hyperthermophilic Archaea Oligoribonuclease Degradation |
| ISSN号 | 1568-7864 |
| DOI | 10.1016/j.dnarep.2018.03.005 |
| 文献子类 | 期刊论文 |
| 英文摘要 | In cells, degrading DNA and RNA by various nucleases is very important. These processes are strictly controlled and regulated to maintain DNA integrity and to mature or recycle various RNAs. NanoRNase (Nrn) is a 3'exonuclease that specifically degrades nanoRNAs shorter than 5 nucleotides. Several Nrns have been identified and characterized in bacteria, mainly in Firmicutes. Archaea often grow in extreme environments and might be subjected to more damage to DNA/RNA, so DNA repair and recycling of damaged RNA are very important in archaea. There is no report on the identification and characterization of Nrn in archaea. Aeropyrum pernix encodes three potential Nrns: NrnA (Ape1437), NrnB (Ape0124), and an Nrn-like protein Ape2190. Biochemical characterization showed that only Ape0124 could degrade ssDNA and ssRNA from the 3'-end in the presence of Mn2+. Interestingly, unlike bacterial Nrns, Ape0124 prefers ssDNA, including short nanoDNA, and degrades nanoRNA with lower efficiency. The 3'-DNA backbone was found to be required for efficiently hydrolyzing the phosphodiester bonds. In addition, Ape0124 also degrads the 3'-overhang of double-stranded DNA. Interestingly, Ape0124 could hydrolyze pAp into AMP, which is a feature of bacterial NrnA, not NrnB. Our results indicate that Ape0124 is a novel Nrn with a combined substrate profile of bacterial NrnA and NrnB. |
| 语种 | 英语 |
| WOS记录号 | WOS:000432617300008 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/29080]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Deng, YJ 2.Feng, L 3.Zhou, H 4.Xiao, X 5.Wang, FP 6.Liu, XP |
| 推荐引用方式 GB/T 7714 | Deng, YJ,Feng, L,Zhou, H,et al. NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA[J]. DNA REPAIR,2018,65:54-63. |
| APA | Deng, YJ,Feng, L,Zhou, H,Xiao, X,Wang, FP,&Liu, XP.(2018).NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA.DNA REPAIR,65,54-63. |
| MLA | Deng, YJ,et al."NanoRNase from Aeropyrum pernix shows nuclease activity on ssDNA and ssRNA".DNA REPAIR 65(2018):54-63. |
入库方式： OAI收割
来源：上海应用物理研究所
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