One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles
文献类型：期刊论文
| 作者 | Li, C. X.; J. Zuo; Q. Q. Li; Y. L. Chang; Y. L. Zhang; L. P. Tu; X. M. Liu; B. Xue; H. Y. Zhao; H. Zhang and X. G. Kong |
| 刊名 | Biosensors & Bioelectronics
 |
| 出版日期 | 2017 |
| 卷号 | 92 |
| 英文摘要 | Despite their general clinical applications, current fluorescence-based immunoassays are confronted with serious challenges, e.g. the advance serum/ plasma separation and the tedious washing process in current heterogeneous approaches, and aggregation of particles, low sensitivity and the narrow linear range in homogeneous approaches. In this paper, these urgent problems were solved in a novel one-step in situ immunoassay of whole blood samples by combining the traditional fluorescence resonance energy transfer (FRET) technology (between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)) and the solid-substrate based immunoassay technology. The low detection limits of goat IgG (gIgG) as 0.042 mu g/mL in buffers, 0.51 mu g/mL in 20-fold diluted whole blood samples and a wide linear range from 0.75 mu g/mL to 60 mu g/mL in blood samples were achieved. To the best of our knowledge, it is the first one-step in situ solid-substrate based immunoassay of whole blood samples with large linear detection range. This development provides a promising platform for a rapid and sensitive immunoassay of various bio-molecules directly in whole blood without tedious separation, washing steps and aggregation problems. |
| 语种 | 英语 |
| 源URL | [http://ir.ciomp.ac.cn/handle/181722/58989]  |
| 专题 | 长春光学精密机械与物理研究所_中科院长春光机所知识产出 |
| 推荐引用方式 GB/T 7714 | Li, C. X.,J. Zuo,Q. Q. Li,et al. One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles[J]. Biosensors & Bioelectronics,2017,92. |
| APA | Li, C. X..,J. Zuo.,Q. Q. Li.,Y. L. Chang.,Y. L. Zhang.,...&H. Zhang and X. G. Kong.(2017).One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles.Biosensors & Bioelectronics,92. |
| MLA | Li, C. X.,et al."One-step in situ solid-substrate-based whole blood immunoassay based on FRET between upconversion and gold nanoparticles".Biosensors & Bioelectronics 92(2017). |
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