Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning.
文献类型：期刊论文
| 作者 | Qiu, Xue; Guo, Jiajia; Jin, Zongwen; Petreto, Alexandra; Medintz, Igor L.; Hildebrandt, Niko |
| 刊名 | SMALL
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Multiplexed photoluminescence (PL) detection plays an important role in chemical and biological sensing. Here, it is shown that time-gated (TG) detection of a single terbium-donor-based Forster resonance energy transfer (FRET) pair can be used to selectively quantify low nanomolar concentrations of multiple DNAs or microRNAs in a single sample. This study demonstrates the applicability of single-TG-FRETpair multiplexing for molecular (Tb-to-dye) and nanoparticle (Tb-to-quantum-dot) biosensing. Both systems use acceptor-sensitization and donor-quenching for quantifying biomolecular recognition and modification of the donor-acceptor distance for tuning the PL decays. TG intensity detection provides extremely low background noise and a quick and simple one-step assay format. Single-TG-FRET-pair multiplexing can be combined with spectral and spatial resolution, paving the way for biosensing with unprecedented high-order multiplexing capabilities. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12023]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | SMALL |
| 推荐引用方式 GB/T 7714 | Qiu, Xue,Guo, Jiajia,Jin, Zongwen,et al. Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning.[J]. SMALL,2017. |
| APA | Qiu, Xue,Guo, Jiajia,Jin, Zongwen,Petreto, Alexandra,Medintz, Igor L.,&Hildebrandt, Niko.(2017).Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning..SMALL. |
| MLA | Qiu, Xue,et al."Multiplexed Nucleic Acid Hybridization Assays Using Single-FRET-Pair Distance-Tuning.".SMALL (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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