DIRECT PCR AMPLIFICATION AND IN SITU IMAGING BASED ON ALGINATE DROPLETS.
文献类型：会议论文
| 作者 | Li, Lijun; Yu, Linfen; Wu, Tianzhun |
| 出版日期 | 2017 |
| 会议日期 | 2017 |
| 会议地点 | Las Vegas, NV |
| 英文摘要 | A droplet based microfluidic system encapsulating PCR reagent into alginate beads as a new digital PCR solution has been developed, which avoided droplet coalescence and enabled efficient DNA amplification and high-throughput fluorescent imaging. We also compared two trapping methods using hydrodynamic traps and micro-sieves, and indicated the advantages of latter for higher trapping rate and no droplet clogging. These results provide new insights and tools foron-chip digital PCR and are expected to attract broad interest for applications in droplet microfluidics. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12209]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2017 |
| 推荐引用方式 GB/T 7714 | Li, Lijun,Yu, Linfen,Wu, Tianzhun. DIRECT PCR AMPLIFICATION AND IN SITU IMAGING BASED ON ALGINATE DROPLETS.[C]. 见:. Las Vegas, NV. 2017. |
入库方式： OAI收割
来源：深圳先进技术研究院
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