A Fiber integrated optofluidic platform for sensitive microRNA FRET detection
文献类型：会议论文
| 作者 | Hongtao Feng; Yi Chen; Lin Liu; Weiliang Shu; Baoyue Zhang; Zongwen Jin; Yan Chen |
| 出版日期 | 2017 |
| 会议日期 | 2017 |
| 会议地点 | Singapore |
| 英文摘要 | We demonstrated a fiber integrated optofluidic platform to perform miRNA detection with high sensitivity and specificity based on FRET principle. Recently miRNA has attracted extensive interest as a biomarker, providing important evidence for early disease diagnosis and post treatment. Traditionally, numerous analytical approaches such as northern blotting, micro arrays , and real-time quantitative polymerase chain reaction have been developed for miRNA detection. However, the existing methods have many technical challenges, for example, the concentration of miRNA in vivo is normally at low level, and the short sequence and easy degradation by RNAase makes it difficult for PCR. Therefore, we developed an innovative optofluidic platform that combines the advantage of droplet microfluidics and optofluidic technology, and provides a low-cost, compact and high specificity approach for identification and quantification of miRNA using FRET principle. Single-nucleotide differences within miRNA family can be distinguished by a novel Y-junction design of donor, receptor and miRNA sequence. We performed one step miRNA detection with simple device operation, and our platform achieved target miRNA identification at a low concentration and high specificity. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12223]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2017 |
| 推荐引用方式 GB/T 7714 | Hongtao Feng,Yi Chen,Lin Liu,et al. A Fiber integrated optofluidic platform for sensitive microRNA FRET detection[C]. 见:. Singapore. 2017. |
入库方式： OAI收割
来源：深圳先进技术研究院
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