Psoralidin promotes osteogenesis and inhibits adipogenesis via genomic and cellular signaling pathway of estrogen receptor
文献类型：会议论文
| 作者 | Huijuan Cao; Cairong Li; Linying Wang; Yuxiao Lai; Ling Qin; Xinluan Wang |
| 出版日期 | 2017 |
| 会议日期 | 2017 |
| 会议地点 | 新疆石河子 |
| 英文摘要 | Objective:This studyaimedto investigate the molecular mechanism of psoralidin on promoting osteogenesis and inhibitingadipogenesis. Methods:Preosteoblasts MC3TC-E1 cells andpreadipocyte 3T3-L1 cells were used in this study. ICI 182,780 (ICI) as a specific antagonist of estrogen receptor (ER) was used to block ER signaling in these two cell lines.Alizarin Red S and Oil Red O staining were used to evaluate the effects of psoralidin on promoting mineralization and inhibiting oil droplets accumulation, respectively. During differentiation processes, the osteogenic- or adipogenesis-related markers were detected. Results:Psoralidin could enhance calcium nodule formation through up-graduatingalkaline phosphataseactivity and osteocalcinlevel. As to adipogenesis, psoralidin decreased adipocytes formation and accumulation through decliningperoxisome proliferator activated receptor γ, CCAAT-enhancer-binding protein α, lipoprotein lipaseandfatty acid binding protein 4mRNA levels. Meanwhile, we found fat-related factorsadiponectin, resistinand leptin levels were all decreased withpsoralidin treatment. ICI could completely block the effect of psoralidin on promoting calcium nodule formation, but only contribute partialeffect on inhibiting adipogenesis. G-15 (a selective G protein-coupled receptor 30 antagonist) and ICI had similar efficiency on blocking the effect ofpsoralidinon inhibiting adipogenesis, yet G-1(a selective G protein-coupled receptor 30 agonist) could not affect the function of psoralidinon promotingmineralization. Conclusion: Psoralidinexhibited similar efficiency and mechanism withestradiol on promoting osteogenesis and inhibiting adipogenesis. Meanwhile, psoralidin promoted maturation and mineralization of MC3T3-E1 cells via mediating ER related classical genomic signaling events, whilepsoralidininhibited adipocyte differentiation and maturation of 3T3-L1 cells via rapid cellular signaling events of estrogen receptor. Acknowledgements:This work was supported by National Nature Scicence Foundation of China (81302782), Ministry of Science and Technology (2015DFG32200), Shenzhen Science and Technology Innovation Committee (GJHZ20150316143827260). |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12250]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2017 |
| 推荐引用方式 GB/T 7714 | Huijuan Cao,Cairong Li,Linying Wang,et al. Psoralidin promotes osteogenesis and inhibits adipogenesis via genomic and cellular signaling pathway of estrogen receptor[C]. 见:. 新疆石河子. 2017. |
入库方式： OAI收割
来源：深圳先进技术研究院
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