In Situ Bioorthogonal Metabolic Labeling for Fluorescence Imaging of Virus Infection In Vivo.
文献类型：期刊论文
| 作者 | Yao, Xiang-jie; Li, Wen-jun; Wu, Ya-yun; Liu, Lan-lan; Pan, Hong; He, Hua-mei; Zhang, Ren-li; Ma, Yi-fan; Cai, Lin-tao |
| 刊名 | SMALL
 |
| 出版日期 | 2017 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Optical fluorescence imaging is an important strategy to explore the mechanism. of virus host interaction. However, current fluorescent tag labeling strategies often. dampen viral infectivity. The present study explores an in situ fluorescent labeling strategy in order to preserve viral infectivity and precisely monitor viral infection in vivo. in contrast to pre-labeling strategy, mice are first intranasally infected with azide-modified H5N1 pseudotype virus (N-3-H5N1p), followed by injection of dibenzocyclooctyl (DBCO)-fiinctionalized fluorescence 6 h later. The results show that DBCO dye directly conjugated to N-3-H5N1p in lung tissues through in vivo bioorthogonal chemistry with high specificity and efficacy. More remarkably, in. situ labeling rather than conventional prelaheling strategy effectively preserves viral infectivity and immunogenicity both in vitro and in vivo. Hence, in situ bioorthogonal viral labeling is a promising and reliable strategy for imaging and tracking viral infection in vivo. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/12253]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | SMALL |
| 推荐引用方式 GB/T 7714 | Yao, Xiang-jie,Li, Wen-jun,Wu, Ya-yun,et al. In Situ Bioorthogonal Metabolic Labeling for Fluorescence Imaging of Virus Infection In Vivo.[J]. SMALL,2017. |
| APA | Yao, Xiang-jie.,Li, Wen-jun.,Wu, Ya-yun.,Liu, Lan-lan.,Pan, Hong.,...&Cai, Lin-tao.(2017).In Situ Bioorthogonal Metabolic Labeling for Fluorescence Imaging of Virus Infection In Vivo..SMALL. |
| MLA | Yao, Xiang-jie,et al."In Situ Bioorthogonal Metabolic Labeling for Fluorescence Imaging of Virus Infection In Vivo.".SMALL (2017). |
入库方式： OAI收割
来源：深圳先进技术研究院
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